Kappa light chain Rat mAb (Biotin) - Primary antibody, specific to Kappa light chain

Cat. No.: Ab190223

GRADE & PURITY Validated, ExactAb™, 0.5 mg/mL

Application

Flow Cytometry

Host species

Rat

Reactive species

Mouse

Isotype

Rat IgG1

Clone Number

187.1 (HB-58)

Conjugation

Biotin

Overview

Rat anti Mouse Kappa light chain Antibody, Monoclonal (187.1 (HB-58)), could be used for Flow

Specifications

Product Name

Kappa light chain Rat mAb (Biotin) - Primary antibody, specific to Kappa light chain

Synonyms

HCAK 1 antibody | HCAK1 antibody | Ig kappa chain C region antibody | IGKC antibody | IGKCD antibody | Immunoglobulin InV antibody | Immunoglobulin kappa constant antibody | Immunoglobulin kappa constant region antibody | Immunoglobulin kappa light chain

Specifications & Purity

ExactAb™, Validated, 0.5 mg/mL

Host species

Rat

Specificity

Kappa light chain

Immunogen

Mouse IgG2b Isotype control antibody clone MPC-11

Positive Control

Flow: C57BL/6 mouse splenocytes

Conjugation

Biotin

Grade

ExactAb™, Validated

Light Chain Type

kappa

Purification Method

Protein G purified

Concentration

0.5 mg/mL

Storage and Shipping

Storage

Store at 2-8°C,Protected from light,Do not freeze

Shipped In

Wet ice,Do not freeze

Stability And Storage

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Images

Flow Cytometry: Kappa light chain Rat mAb (Biotin) (Ab190223)

Kappa light chain Rat mAb (Biotin) (Ab190223) - Flow Cytometry
Flow cytometry analysis of kappa light chain expression by C57BL/6 mouse splenocytes (right panel) compared to an isotype control (left panel). Cells were stained with Kappa light chain Rat mAb (Biotin) (Ab190223) at 0.1μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Left panel - Rat IgG (Biotin) (Ab180310). 

Flow Cytometry: Kappa light chain Rat mAb (Biotin) (Ab190223)

Kappa light chain Rat mAb (Biotin) (Ab190223) - Flow Cytometry
Flow cytometry analysis of kappa light chain expression by C57BL/6 mouse splenocytes (red) compared to an isotype control (blue). Cells were stained with Kappa light chain Rat mAb (Biotin) (Ab190223) at 0.1μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Blue - Rat IgG (Biotin) (Ab180310). Black - Unlabelled control, cells without incubation with primary antibody.

Application

• Application: Dilution info
• Flow Cytometry:

  ≤1.0μg/test

  For flow cytometric staining, the suggested use of this reagent is ≤1.0 μg per million cells in 100 µL staining volume. ;

Associated Targets(Human)

IGKC Tdark Immunoglobulin kappa constant (0 Activities)

Discover Target: IGKC

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)