GRADE & PURITYCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.Azide Free?Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory.PBS Only?PBS-only formulation — supplied in phosphate-buffered saline with no other additives. Use when you need a clean PBS buffer free of stabilizers/carriers.≥95%(SDS-PAGE)See COA
Carrier Free,Azide Free,Validated,PBS Only,≥95%(SDS-PAGE),See COA Azide Free,Carrier Free,PBS Only,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at 2-8°C,Do not freeze Ships Wet ice,Do not freeze Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Rat anti Mouse PD-L2/CD273 Antibody, Monoclonal (TY25), could be used for in vivo PD-L2 blockade, in vitro PD-L2 blockade, IHC, Flow and so on. Application: in vivo PD-L2 blockade: Use at an assay dependent concentration. in vitro PD-L2 blockade: Use at an assay dependent concentration. IHC: Use at an assay dependent concentration. Flow: Use at an assay dependent concentration. Protein Function Involved in the costimulatory signal, essential for T-cell proliferation and IFNG production in a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation by blocking cell cycle progression and cytokine production.
Specifications
Product Name
PD-L2/CD273 Rat mAb - Primary antibody, specific to PD-L2/CD273, Rat IgG2a
Carrier Free, Azide Free, Validated, PBS Only, ≥95%(SDS-PAGE), See COA
Host species
Rat
Specificity
PD-L2/CD273
Immunogen
Mouse PD-L2 transfected cell line
Conjugation
Unconjugated
Grade
Azide Free, Carrier Free, PBS Only, Validated
Isotype
Rat IgG2a
Light Chain Type
kappa
SDS-PAGE
27.0 kDa (Light Chain) & 52.3 kDa (Heavy Chain), under reducing conditions; 212.1 kDa, under non-reducing conditions.
Purification Method
Protein G purified
Concentration
See COA
Storage and Shipping
Shape
Liquid
Storage
Store at 2-8°C,Do not freeze
Shipped In
Wet ice,Do not freeze
Stability And Storage
Shipped at 4°C. Store at 4°C long term (12 months). Do not freeze. Upon receipt, it is recommended to aliquot.
Images
PD-L2/CD273 Rat mAb (Ab121050) - Flow Cytometry Two-color flow cytometric analysis of PD-L2/CD273 expression on mouse splenocytes. C57BL/6 mouse spleen cells were unstimulated (left) or 10 ng/mL mouse GM-CSF protein (rp154039) stimulated (right), and stained with CD11c Armenian Hamster mAb (APC) (Ab178872) and PD-L2/CD273 Rat mAb (Ab121050) at 0.5 μg/mL. Two-color flow cytometric dot plots showing the correlated expression of CD11C versus PD-L2/CD273 (or Isotype control staining) were derived from gated events with the forward and side light-scatter characteristics of viable mononuclear cells.
Application
Application
Dilution info
IHC
Use at an assay dependent concentration.
Flow Cytometry
Use at an assay dependent concentration.
in vivo PD-L2 blockade
Use at an assay dependent concentration.
in vitro PD-L2 blockade
Use at an assay dependent concentration.
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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Products are supplied to verified businesses, institutions, and qualified professionals for research and development use only. Not for use in humans, animals, diagnosis, or therapy.
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