Recombinant Human EGFR Isoform vIII Protein

Cat. No.: rp176275

GRADE & PURITY Animal Free, Carrier Free, Bioactive, ActiBioPure™, His Tag, PBS Only, ≥95%(SDS-PAGE)

Expression System

HEK293

Accession #

NP_001333870.1

Protein Tag

C-His

Expression system

HEK293

Endotoxin Concentration

<1.0 EU/μg

Bioactivity

Immobilized Recombinant Human EGFR Isoform vIII Protein (rp176275) at 1.0 μg/mL can bind Depatuxizumab mafodotin (anti-EGFR) (Ab175508) with the EC50 of 19.11 ng/mL. Immobilized Recombinant Human EGFR Isoform vIII Protein (rp176275) at 1.0 μg/mL can bind Depatuxizumab (anti-EGFR) (Ab182750) with the EC50 of 22.53 ng/mL.

Overview

Protein Function:
As a member of the epidermal growth factor receptor (EGFR) family, EGFR protein is type I transmembrane glycoprotein that binds a subset of EGF family ligands including EGF, amphiregulin, TGF-α, betacellulin, etc. EGFR protein plays a crucial role in signaling pathway in the regulation of cell proliferation, survival and differentiation. Binding of a ligand induces EGFR protein homo- or heterodimerization, the subsequent tyrosine autophosphorylation and initiates various down stream pathways (MAPK, PI3K/PKB and STAT). In addition, EGFR signaling also has been shown to exert action on carcinogenesis and disease progression, and thus EGFR protein is proposed as a target for cancer therapy currently.

Specifications

Product Name

Recombinant Human EGFR Isoform vIII Protein

Synonyms

avian erythroblastic leukemia viral (v-erb-b) oncogene homolog | cell growth inhibiting protein 40 | cell proliferation-inducing protein 61 | EC 2.7.10 | EC 2.7.10.1 | EGF R | EGFR | epidermal growth factor receptor (avian erythroblastic leukemia viral (v

Grade

ActiBioPure™, Animal Free, Bioactive, Carrier Free, His Tag, PBS Only

Specifications & Purity

Animal Free, Carrier Free, Bioactive, ActiBioPure™, His Tag, PBS Only, ≥95%(SDS-PAGE)

Bioactivity

Immobilized Recombinant Human EGFR Isoform vIII Protein (rp176275) at 1.0 μg/mL can bind Depatuxizumab mafodotin (anti-EGFR) (Ab175508) with the EC50 of 19.11 ng/mL. Immobilized Recombinant Human EGFR Isoform vIII Protein (rp176275) at 1.0 μg/mL can bind Depatuxizumab (anti-EGFR) (Ab182750) with the EC50 of 22.53 ng/mL.

Endotoxin Concentration

<1.0 EU/μg

Expression System

HEK293

Amino Acids

1-378 aa

Sequence

MRPSGTAGAALLALLAALCPASRALEEKKGNYVVTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNGIGIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTVKEITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEISDGDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCWGPEPRDCVSCRNVSRGRECVDKCNLLEGEPREFVENSECIQCHPECLPQAMNITCTGRGPDNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLEGCPTNGPKIPSHHHHHH

Protein Tag

C-His

Accession #

NP_001333870.1

Predicted molecular weight

41.7 kDa

SDS-PAGE

56.2-67.2 kDa, under reducing conditions

Molecule Type

Protein

Storage and Shipping

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 0.5 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at -20°C for 1 year. Avoid freeze / thaw cycle.

Images

ELISA: Recombinant Human EGFR Isoform vIII Protein (rp176275)

Recombinant Human EGFR Isoform vIII Protein (rp176275) - ELISA
Immobilized Recombinant Human EGFR Isoform vIII Protein (rp176275) at 1.0 μg/mL can bind Depatuxizumab mafodotin (anti-EGFR) (Ab175508) with the EC50 of 19.11 ng/mL.

ELISA: Recombinant Human EGFR Isoform vIII Protein (rp176275)

Recombinant Human EGFR Isoform vIII Protein (rp176275) - ELISA
Immobilized Recombinant Human EGFR Isoform vIII Protein (rp176275) at 1.0 μg/mL can bind Depatuxizumab (anti-EGFR) (Ab182750) with the EC50 of 22.53 ng/mL.

SDS-PAGE: Recombinant Human EGFR Isoform vIII Protein (rp176275)

Recombinant Human EGFR Isoform vIII Protein (rp176275) - SDS-PAGE
3 μg/lane of Recombinant Human EGFR Isoform vIII Protein was resolved with SDS-PAGE under reducing (R) conditions and visualized by Coomassie® Blue staining, showing a band at 56.2-67.2 kDa under reducing conditions.