Streptavidin and Conjugates, CAS No.9013-20-1

CAS: 9013-20-1 Cat. No.: S103034 EC Number: 618-481-6 PubChem CID: 51062757

GRADE & PURITY from Streptomyces avidinii,≥12 U/mg protein

Overview

Streptavidin and Conjugates include streptavidin in a purified form, conjugated to enzymes or fluorophores or activated for crosslinking to sulfhydryl or carbonyl groups. These purified and conjugated forms of recombinant streptavidin protein enable detection of biotinylated antibodies and other probes in a variety of standard assay methods, including Western blotting, ELISA, immunohistochemistry and fluorescence imaging. Purified streptavidin and the streptavidin-HRP conjugates (peroxidase-conjugated streptavidin) are supplied lyophilized in phosphate-based buffers for immediate reconstitution with water. Alkaline Phosphatase conjugates are supplied as a liquid in a Tris-based buffer.
Applications for enzyme-labeled (HRP, AP) streptavidin: Enzyme immunoassays (EIA), including enzyme-linked immunsorbent assays (ELISA) with biotinylated antibodies Cell and tissue staining (for light microscopy) Western blotting (WB) and other membrane-based biotin detection Applications for fluorescent (FITC, TRITC or Texas Red* Fluor-labeled) streptavidin: Immunofluorescent assays (IFA) Tissue staining (for fluorescence microscopy) Blot immunostaining Cell staining (incl. fluorescent activated cell sorting)

Specifications

Product Name

Streptavidin and Conjugates, CAS No.9013-20-1

Synonyms

EX-A4899 | CID 51062757 | FT-0700692 | 2-[(3-chlorophenyl)-piperidin-1-ium-1-ylidenemethyl]sulfanylacetic acid;bromide

Specifications & Purity

from Streptomyces avidinii, ≥12 U/mg protein

Biochemical and Physiological Mechanisms

reptavidin is biotin-binding protein that was originally isolated from Streptomyces avidinii. In contrast to avidin, streptavidin has no carbohydrate and has a mildly acidic pI of 5. OursStreptavidin products use a recombinant form of streptavidin having

Conjugation

Unconjugated

Note

Source recombination, including HIS tags

CAS

9013-20-1

Molecule Type

Protein

Storage and Shipping

Concentration

from Streptomyces avidinii,≥12 U/mg protein

Storage

Store at -20°C,Argon charged

Shipped In

Ice chest + Ice pads

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66. Yu-E Gao, Qing Huang, Yue Huang, Li-Xia Yan, Wen-Long Wang, WenFeng Zhao, Yong-Wei Feng, Xiao-Dong Huang, Yi Zhang.  (2024)  Nanoparticle-mediated light-driven LAMP combined with test strips for sensitive and rapid visual detection of antibiotic resistance genes.  JOURNAL OF HAZARDOUS MATERIALS,      [PMID:39752829] [10.1016/j.jhazmat.2024.136981]

67. Wenli Zhu, Miao Hu, Guanghua Su, Weifang Jiang, Hancong Du, Kaxi Chen, Jinfang Nie, Lang Zhang, Xuehui Tang, Yun Zhang.  (2025)  Nanoprobe-free nanosensor with Tyndall-effect readout for colorimetric detection of alkaline phosphatase.  MICROCHEMICAL JOURNAL,      [PMID:] [10.1016/j.microc.2025.113295]

68. Liping Wu, Min Qing, Zhaode Mu, Jing Zhou, Jianli Zuo, Lijuan Bai.  (2024)  New electrocatalyst of Ni6MnO8@AB/PtNPs with efficient synergistic enhancement in electrochemical aptasensor for sensitive detection of neuron specific enolase.  MICROCHEMICAL JOURNAL,      [PMID:] [10.1016/j.microc.2024.110639]

69. Huanxin Xiao, Weiguang Chen, Mingxia Lin, Shilin Jiang, Xiping Cui, Suqing Zhao.  (2024)  Rapid immunoassay for dual-mode detection of HPV16 and HPV18 DNA based on Au@PdPt nanoparticles.  Analytical Methods,      [PMID:38463013] [10.1039/D3AY02307A]

70. Jianjian Ji, Yingcai Xiong, Keyu Tao, Tao Li, Weiying Ou, Yinghui Zhou, Wenyang Zhang, Ruogu Qi, Shouchuan Wang.  (2024)  Resveratrol inhibits respiratory syncytial virus replication by targeting heparan sulfate proteoglycan.  Food & Function,      [PMID:38270052] [10.1039/D3FO05131E]

71. Ziru Feng, Mengmeng Yan, Tengfei Li, Wenjun Zhang, Chao Zhu.  (2025)  Screening specific aptamer pairs towards bovine lactoferrin for its sandwich application.  Food Bioscience,      [PMID:] [10.1016/j.fbio.2025.105911]

72. Jialuo Ai, Yixuan Huang, Zhaoyi Yin, Yingshan Deng, Ling Yan, Jingwen Liao, Guoyan Liang, Chong Chen, Yunbing Chang, Cairong Xiao, Jiale Zhou, Zurong Zhu, Chengli Liu, Zhuo Jiang, Chengyun Ning, Zhengao Wang.  (2024)  SEA Anemone-inspired Conducting Polymer Sensing Platform for Integrated Detection of Tumor Protein Marker and Circulating Tumor Cell.  Advanced Healthcare Materials,      [PMID:38767216] [10.1002/adhm.202401305]

73. Yi Zhang, Jing Cao, Jie Zhou, Wen-Long Wang, Zhenghua Xu, Yong-Wei Feng, Xiao-Dong Huang, Feng-De Yu, Xin Yang.  (2024)  Strand displacement amplification combining photothermal-colorimetric dual mode test strip for ultra-sensitive detection of patulin.  MICROCHEMICAL JOURNAL,      [PMID:] [10.1016/j.microc.2024.112109]

74. Qiang Zhang, Junlei Zhao, Anqi Han, Xiaonan Zhang, Mingya Yang, Hui Li, Benli Yu, Guosheng Zhang, Sheng Zhou.  (2024)  Ultrasensitive cancer cell sensing based on tapered optical fiber operating near the dispersion turning point.  SENSORS AND ACTUATORS B-CHEMICAL,      [PMID:] [10.1016/j.snb.2024.135473]

75. Songbai Tian, Tingting Xiang, Xinghu Ji, Fuxiang Zhou, Zhike He.  (2025)  Membrane-based microfluidic chip constructed and applied in magnetic microparticle anchoring-and-release digital immunoassay.  TALANTA,      [PMID:40743937] [10.1016/j.talanta.2025.128644]

76. Xiaojuan You, Mingyi Shao, Huadong Wang, Rui Zhu, Xinwei Liu, Lei Dong, Yuesheng Gong, Yongwei Li.  (2025)  An electrochemical aptasensor for detection of Helicobacter pylori based on AuNPs and AgNPs-GO nanoparticles.  Frontiers in Bioengineering and Biotechnology,      [PMID:40704101] [10.3389/fbioe.2025.1619336]

77. Xiru Zhang, Manyan Qiu, Yiyi Zhang, Qianyu Zhao, Bo Qu, Wei Zhang, Xianlong Zhang, Yujun Jiang.  (2025)  Pyrococcus furiosus Argonaute biosensing platform based on hemin@ZIF-90 nanozyme-modulated signal amplification for sensitive detection of Salmonella typhimurium.  FOOD CHEMISTRY,      [PMID:40845695] [10.1016/j.foodchem.2025.145952]

78. Lixia Li, Yurui Huang, Jiabin Zhao, Feiyou Liu, Ning Feng, Yufang Liu.  (2025)  Deep learning-enhanced ultra-sensitive fiber optic SPR sensor for mercury ion detection.  INFRARED PHYSICS & TECHNOLOGY,      [PMID:] [10.1016/j.infrared.2025.106128]

79. Haiyan Zhao, Yunzhi Zhao, Shanshan Li, Meiya Zhang, Na Li, Min Cui, Cong Zhang.  (2025)  Dual-signal ratiometric electrochemical aptasensor based on COFs-derived Fe/N-doped porous carbon and methylene blue-enriched COFs for sensitive and accurate detection of kanamycin.  FOOD CHEMISTRY,      [PMID:41014848] [10.1016/j.foodchem.2025.146517]

80. Zhao Fengxia, Jia Shiyu, Yan Hangli, Bai Qinqin, Hu Hongmei, Liang Hao, Niu Xiangheng.  (2025)  Label-free HCR-facilitated nanozyme catalysis and fluorescence simultaneous amplification for ultrasensitive dual-signal sensing of foodborne Salmonella typhimurium.  MICROCHIMICA ACTA,  192  (10): (1-10).  [PMID:40952515] [10.1007/s00604-025-07510-5]

81. Hanjun Chen, Ying Liu, Shaoqiong Feng, Yu Cao, Tingting Wu, Zhihong Liu.  (2021)  Cotton thread-based multi-channel photothermal biosensor for simultaneous detection of multiple microRNAs.  BIOSENSORS & BIOELECTRONICS,      [PMID:34968855] [10.1016/j.bios.2021.113913]

82. Xia Xu, Xin Liu, Yixing Qiu, Yufan Song, Xuxia Zhou, Yuting Ding.  (2024)  Colorimetric aptasensor based on Fe3O4@MOF@Pt nanozymes for ultrasensitive detection of histamine in fish.  FOOD CONTROL,      [PMID:] [10.1016/j.foodcont.2024.110702]

83. Zhenfeng Yu, Jialing Zhou, Zi-Han Chen, Zhihua Wang, Aliyah Almomen, Ahmed Mohamed El-Toni, Aibin Liang, Yong Fan, Fan Zhang.  (2025)  A Multifunctional NIR-II Rare-Earth Nanoagent for In Vivo Monitoring Cancer Revascularization and Prognosis.  ADVANCED FUNCTIONAL MATERIALS,      [PMID:] [10.1002/adfm.202527382]

84. Shun Hu, Liding Zhang, Ying Su, Xiaohan Liang, Jie Yang, Qingming Luo, Haiming Luo.  (2024)  Sensitive detection of multiple blood biomarkers via immunomagnetic exosomal PCR for the diagnosis of Alzheimer’s disease.  Science Advances,  10  (13):   [PMID:38536917] [10.1126/sciadv.abm3088]

85. Shengen Gu, Jiao Lei, Shuaihang Guo, Jun Sun, Yu Duan, Aiqing Li, Mengying Zhan, Lisha Pan, Feng Zhou, Xiaoli Liu, Hong Chen.  (2025)  Renewable and Switchable Biofunctional Modification of Poly(dimethylsiloxane) Surfaces via Host–Guest Interactions for Enhanced Capture of Circulating Tumor Cells in Microfluidics.  ACS Applied Materials & Interfaces,      [PMID:40257044] [10.1021/acsami.5c00707]

86. Lixia Li, Jiabin Zhao, Mingdeng Jin, Siyuan Wu, Feiyou Liu, Yurui Huang, Ning Feng, Yufang Liu.  (2025)  Indium tin oxide/gold/graphene oxide composite membrane structure and gold nanoparticles synergistically enhanced fiber optic sensor for mercury ion detection.  MEASUREMENT,      [PMID:] [10.1016/j.measurement.2025.117735]

87. Lianpeng Lv, Xiaojing Kang, Lubin Hua, Zejian Du, Ruixue Sun, Yefeng Deng, Hailiang Yang, Zheng Xu, Yang Zhou, Bing Wang.  (2025)  Sandwich-type electrochemical immunosensor based on MXene/CNTs substrate and MOFs (MIL-101) probe for leather artifact identification.  MICROCHEMICAL JOURNAL,      [PMID:] [10.1016/j.microc.2025.113810]

88. Haowei Dong, Jingcheng Huang, Zhen Guo, Haifang Wang, Lingjun Geng, Ziping Cai, Ibrahim A. Darwish, Yemin Guo, Xia Sun, Yanfang Wu.  (2025)  Prussian blue nanoparticles-based signal amplification in aptamer-based lateral flow assay for procymidone detection.  JOURNAL OF FOOD COMPOSITION AND ANALYSIS,      [PMID:] [10.1016/j.jfca.2025.107756]

89. Weiming Lin, Tao Ding, Die He, Nan Zhang, Haodong Li, Wenjian Luo, Zhongxia Wei, Min Ke, Sisi Jia, Chunhai Fan, Le Liang.  (2025)  DNA Logic-Integrated Quantum Nanosensor for MicroRNA Diagnostics.  JACS Au,      [PMID:40443878] [10.1021/jacsau.5c00058]

90. Lixia Li, Yurui Huang, Siyuan Wu, Mingdeng Jin, Jiabin Zhao, Feiyou Liu, Yiyang Shen, Ning Feng, Yufang Liu.  (2025)  Metal-organic frameworks/graphene oxide synergistic enhanced optical fiber SPR sensor for ultra-trace lead ions detection.  OPTICS AND LASERS IN ENGINEERING,      [PMID:] [10.1016/j.optlaseng.2025.109115]

91. Yixuan Yang, Xia Liu, Lanlan Jia, Jiabo Wang, Qianhui Wu, Minglei Zhang, Yusi Bu, Xiaoyu Xie.  (2025)  Leukocyte Membrane-Coated Filtrable Micromotors With Selective Distribution of Enzymes for Capturing and Sensing Circulating Tumor Cells.  Small,      [PMID:] [10.1002/smll.202507095]

92. Jiang Wenxin, Du Huanmin, Huang Xingjie, Lee Luke P., Chen Chia-Hung.  (2025)  Sensitive, high-throughput, metabolic analysis by molecular sensors on the membrane surface of mother yeast cells.  Nature Communications,  16  (1): (1-14).  [PMID:41057294] [10.1038/s41467-025-63964-4]

93. Kun Fu, Jia Zheng, Yichao Zhang, Chang Liu, Wenxiang Zhang, Siyu Chen.  (2025)  Construction of a multiplex biosensor for the on-site rapid detection of phthalates in biological fluids based on single-stranded DNA aptamers.  JOURNAL OF HAZARDOUS MATERIALS,      [PMID:41109023] [10.1016/j.jhazmat.2025.140164]

94. Meiyun Shang, Juan Hu, Mengjia Shi, Yunpeng Fan, Yuan Zou, Yong Wang, Jinhong Guo, Cuiping Mao.  (2025)  Zr-MOF encapsulated-magnetic beads-based fast and efficient conjugation strategy for sensitive immunoassays and early diagnosis of rheumatoid arthritis.  MICROCHEMICAL JOURNAL,      [PMID:] [10.1016/j.microc.2025.115926]

95. Zheng Liu-Ting, Yan Zeng-Shuai, Li Xin-Yue, Chang Jia-Jia, Tan Xiao-Qi, Wang Yu-Xing, Ding Hong-Ming, Liu Qin, Ma Yu-Qiang, Huo Da.  (2025)  Nanomaterial signatures program biomolecular condensates via triphasic separation for chemoplasticity remodeling.  Nature Communications,  16  (1): (1-24).  [PMID:41162367] [10.1038/s41467-025-64623-4]

96. Xin Yu, Jingzhang Wang, Guanghao Huang, Lianqun Zhou, Zhenjie Zhang, Shuzhi Sam Ge, Qing Wang, Hui Kong.  (2025)  Highly sensitive detection of C-reactive protein in sweat via dendritic gold structure with double-antibody sandwich assay.  SENSORS AND ACTUATORS B-CHEMICAL,      [PMID:] [10.1016/j.snb.2025.139180]

97. Weixuan Liu, Wen Yan, Lingmei Niu.  (2025)  A dual-target fluorescent assay for rapid detection of foodborne pathogens based on aptamer–magnetic bead technology.  Analytical Methods,      [PMID:41328792] [10.1039/D5AY01696G]

98. Liu-Feng Yu, Jie Zhou, Jing Cao, Bing-Huang Zhu, Yue Huang, Li-Xia Yan, Xiao-Dong Huang, Yi Zhang, Wei Gao.  (2025)  Dual-functional Cu2-xSe nanoparticles enabled photothermal SDA-LFA for sensitive miRNA-146a on-site detection in dairy safety.  TALANTA,      [PMID:41406696] [10.1016/j.talanta.2025.129265]

99. Baiqi Cui, Xiaoyin Liu, Jinbiao Ma, Jingyu Wu, Yunxiao Wang, Haiying Ding, Di Wang, Qingjun Liu, Fenni Zhang.  (2025)  Phase-Sensitive Surface Plasmon Resonance Imaging with Polarization Modulation and Stokes Vector Measurement.  ACS Sensors,      [PMID:41035361] [10.1021/acssensors.5c02048]

100. Li Yixian, Yang Xiaohui, Cheng Shanshan, Mu Rongrong, Li Huiqiang, Yu Yang.  (2025)  A bidirectional immuno-microfluidic chip for detecting autoantibodies based on an indirect assay.  MICROCHIMICA ACTA,  193  (1): (28).  [PMID:41405727] [10.1007/s00604-025-07743-4]

101. Xiaoshuo Li, Xinyue Ma, Zhuolin Fu, Zongwen Jin, Chunyan Sun, Xue Qiu, Jiajia Guo.  (2026)  Ultrasensitive duplex lateral flow immunoassay for enhanced detection of viral antigens using hybridization chain reaction amplification.  MICROCHEMICAL JOURNAL,      [PMID:] [10.1016/j.microc.2026.117144]

102. Chuwen Song, Rong Huang, Peiyong Song, Ke Shi, Jianming Li, Yiyang Lin.  (2026)  Engineering Nucleobase-Functionalized Bottlebrush Polymers as Multivalent Ligands for the Surface Protection of Colloidal Nanomaterials.  ACS Applied Nano Materials,      [PMID:] [10.1021/acsanm.5c05026]

103. Qizhi Diao, Sha Tang, Xin Liu, Jiajia Li, Xiangmin Zhou, Yuanyu Chen, Qiongyuan Zhang, Fangyu Yang.  (2026)  LAMP-LFD: establishment and evaluation of a new diagnostic platform for SARS-CoV-2 rapid detection.  Frontiers in Public Health,      [PMID:41952823] [10.3389/fpubh.2026.1798338]

104. Tingling Lin, Shiqing Li, Yi Huang, Shuncong Zhong, Fuwei Sun, Yujie Zhong, Qiuming Zeng, Rongkun Yuan, Yonglin Huang.  (2026)  Intense terahertz field confined metallic nanoarchitectures for targeted actively capturing and specific biosensing via multi-step surface functionalization.  SENSORS AND ACTUATORS B-CHEMICAL,      [PMID:] [10.1016/j.snb.2026.139794]

105. Wanqi Zhang, Lu Han, Yanhao Yin, Yaoting Mou, Yuhang Tian, Zhicong Sun, Yanfang Wu, Dongfei Chen, Yemin Guo, Xia Sun, Falan Li.  (2026)  Dual signal amplification-based lateral flow strip for rapid and sensitive detection of streptomycin in milk.  Food Bioscience,      [PMID:] [10.1016/j.fbio.2026.108646]

106. Xinwei Zhang, Chonglu Li, Mingzhe Yan, Yuting Wang, You Dou, Tuotuo Zhang, Shuo Zheng, Hanying Wang, Junrong Li, Yao Sun.  (2026)  Molecularly Engineered SERS Platform with Microturbulence-Enhanced Electrohydrodynamics for Multiplexed Profiling of Lung Cancer ctDNA.  Journal of the American Chemical Society,      [PMID:] [10.1021/jacs.6c01526]

107. Qing Kang, Yidi Liu, Xiubing Cui, Jianming Chu, Tong Lin, Jingbo Jiao, Xinjun Du, Shuo Wang.  (2026)  A Multivalent Aptamer-Driven “4 + 1” Nanozyme Platform for Ultrasensitive Detection and Synergistic Inactivation of Salmonella typhimurium.  ANALYTICAL CHEMISTRY,      [PMID:] [10.1021/acs.analchem.5c08087]