CD16/CD32 Rat mAb (Biotin) - Primary antibody, specific to CD16/CD32, Rat IgG2b

Cat. No.: Ab179681

GRADE & PURITY Validated, ExactAb™, Azide Free, 0.5 mg/mL

Application

Flow Cytometry

Host species

Rat

Reactive species

Mouse

Isotype

Rat IgG2b

Clone Number

2.4G2

Conjugation

Biotin

Overview

Application:
Flow: ≤0.5μg/test
For flow cytometric staining, the suggested use of this reagent is ≤0.5 μg per million cells in 100 µL staining volume or 100 µL of whole blood.

Specifications

Product Name

CD16/CD32 Rat mAb (Biotin) - Primary antibody, specific to CD16/CD32, Rat IgG2b

Synonyms

CD16 | CD16A | CD16-II | FCG3 | FCGR3 | FCGR3A | FCGRIII | FcGRIIIA | FCR-10 | FCRIII | FCRIIIA | IGFR3 | IMD20 | Low affinity immunoglobulin gamma Fc region receptor III-A | CD32/CD16 | Fc gamma RII/RIII | CD16a antibody | CD16b antibody | CD32 antibody

Specifications & Purity

ExactAb™, Validated, Azide Free, 0.5 mg/mL

Host species

Rat

Specificity

CD16/CD32

Immunogen

Mouse BALB/c Macrophage J774

Positive Control

Flow: Murine splenocytes.

Conjugation

Biotin

Grade

Azide Free, ExactAb™, Validated

Isotype

Rat IgG2b

Purification Method

Protein G purified

Concentration

0.5 mg/mL

Storage and Shipping

Shape

Liquid

Storage

Store at 2-8°C,Protected from light,Do not freeze

Shipped In

Wet ice,Do not freeze

Stability And Storage

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Images

Flow Cytometry: CD16/CD32 Rat mAb (Biotin) (Ab179681)

CD16/CD32 Rat mAb (Biotin) (Ab179681) - Flow Cytometry
Flow cytometry analysis of CD16/CD32 expression by mouse splenocytes cells (right panel) compared to an isotype control (left panel). Cells were stained with CD16/CD32 Rat mAb (Biotin) (Ab179681) at 0.03μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Left panel - Rat IgG Isotype Control Antibody (Biotin) (Ab180310).

Flow Cytometry: CD16/CD32 Rat mAb (Biotin) (Ab179681)

CD16/CD32 Rat mAb (Biotin) (Ab179681) - Flow Cytometry
Flow cytometry analysis of CD16/CD32 expression by mouse splenocytes cells (red) compared to an isotype control (blue). Cells were stained with CD16/CD32 Rat mAb (Biotin) (Ab179681) at 0.03μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Blue - Rat IgG Isotype Control Antibody (Biotin) (Ab180310). Black - Unlabelled control, cells without incubation with primary antibody.

Application

• Application: Dilution info
• Flow Cytometry:

  ≤0.5μg/test

  For flow cytometric staining, the suggested use of this reagent is ≤0.5 μg per million cells in 100 µL staining volume.;