GRADE & PURITY Validated, 0.5 mg/mL

Application

Flow Cytometry

Host species

Rat

Reactive species

Mouse

Isotype

Rat IgG2b

Clone Number

GK1.5

Conjugation

Biotin

Overview

Application:
Flow: ≤0.5μg/test
For flow cytometric staining, the suggested use of this reagent is ≤0.5 μg per million cells in 100 µL staining volume.

Specifications

Product Name

CD4 Rat mAb (Biotin) - Primary antibody, specific to CD4, Rat IgG2b

Synonyms

CD_antigen: CD4 | CD4 antigen (p55) | CD4 antigen | CD4 molecule | CD4 receptor | CD4 | CD4mut | T-cell surface antigen T4/Leu-3 | T-cell surface glycoprotein CD4 | CD 4 antibody | CD4 (L3T4) antibody | CD4 antibody | CD4 antigen (p55) antibody | CD4 anti

Specifications & Purity

Validated, 0.5 mg/mL

Host species

Rat

Specificity

CD4

Immunogen

Mouse CTL clone V4

Positive Control

Flow: Murine splenocytes.

Conjugation

Biotin

Grade

Validated

Isotype

Rat IgG2b

Purification Method

Protein G purified

Concentration

0.5 mg/mL

Storage and Shipping

Storage

Store at 2-8°C,Protected from light,Do not freeze

Shipped In

Wet ice,Do not freeze

Stability And Storage

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Images

Flow Cytometry: CD4 Rat mAb (Biotin) (Ab181109)

CD4 Rat mAb (Biotin) (Ab181109) - Flow Cytometry
Flow cytometry analysis of CD4 expression by mouse splenocytes cells (right panel) compared to an isotype control (left panel). Cells were stained with CD4 Rat mAb (Biotin) (Ab181109) at 0.1μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Left panel - Rat IgG Isotype Control Antibody (Biotin) (Ab180310).

Flow Cytometry: CD4 Rat mAb (Biotin) (Ab181109)

CD4 Rat mAb (Biotin) (Ab181109) - Flow Cytometry
Flow cytometry analysis of CD4 expression by mouse splenocytes cells (red) compared to an isotype control (blue). Cells were stained with CD4 Rat mAb (Biotin) (Ab181109) at 0.1μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Blue - Rat IgG Isotype Control Antibody (Biotin) (Ab180310). Black - Unlabelled control, cells without incubation with primary antibody.

Application

• Application: Dilution info
• Flow Cytometry:

  ≤0.5μg/test

  For flow cytometric staining, the suggested use of this reagent is ≤0.5 μg per million cells in 100 µL staining volume.;

Associated Targets(Human)

CD4 Tclin T-cell surface glycoprotein CD4 (0 Activities)

Discover Target: CD4

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mechanisms of Action