Collagenase from Clostridium histolyticum, CAS No.9001-12-1

CAS: 9001-12-1 Cat. No.: C754905 EC Number: 232-582-9 PubChem CID: 44384985

GRADE & PURITY purified by chromatography,≥500 CDU/mg solid (CDU = collagen digestion units), lyophilized powder

Accession #

P00791

Overview

Collagenase from Clostridium histolyticum comprises of collagenases G and H. Collagenase has N-terminal activator domain and C-terminal peptidase domain. The collagen binding domains (CBD) and polycystic kidney disease-like (PKD-like) domain resides in the peptidase domain. The collagenase module undergoes structural changes upon calcium binding.

Application:

Collagenase from Clostridium histolyticum has been used:

• for the recovery of neutrophils from collagen gels
• for the dissociation of breast tumor biopsies into single cells
• for the isolation of primary human endometrial stromal cells(HESC) from endometrial biopsies

Collagenase from Clostridium histolyticum may be used for the isolation of cells from many types of animal tissue. It may be used for the disaggregation of human tumor, mouse kidney, human adult/fetal brain, lung and many other tissues. It may also be used in liver and kidney perfusion studies, for the digestion of pancreas, isolation of nonparenchymal rat liver cells and for hepatocyte preparation.

Specifications

Product Name

Collagenase from Clostridium histolyticum, CAS No.9001-12-1

Specifications & Purity

purified by chromatography, ≥500 CDU/mg solid (CDU = collagen digestion units), lyophilized powder

Biochemical and Physiological Mechanisms

Crude collagenases are mixtures of enzymes (mostly proteases) secreted by C. histolyticum. Effective release of cells from tissue requires the action of both collagenase enzymes and the neutral protease. Collagenase is activated by four gram atom calciu

Accession #

P00791

CAS

9001-12-1

Enzyme Commission Number

3.4.24.3(BRENDA, IUBMB)

Molecule Type

Small molecule

Storage and Shipping

Concentration

purified by chromatography,≥500 CDU/mg solid (CDU = collagen digestion units), lyophilized powder

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

References

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