Polyconazole is a widely used plant growth retardant in agriculture, which can be effectively used to cultivate strong crop seedlings and improve plant resistance. This experiment compares the effects of different concentrations of polyconazole on the growth of rice seedlings, to understand and grasp the principles and methods of polyconazole on cultivating strong rice seedlings.
Operation method
Experiments on the seedling-strengthening effect of the plant growth retardant polyazole
Principle
The plant growth retardant polyazole can effectively inhibit the biosynthesis of gibberellin in the plant body, delay the division and elongation of the stem subapical meristematic tissues, thus shortening the internodes without reducing the number of internodes, without affecting the development of the differentiation of the leaves and flowers, and promote the formation of the cell division, but also increase the content of chlorophyll, and improve the efficiency of photosynthesis. As a result, the application of doxorubicin can significantly affect the growth of seedlings, dwarfing seedlings, developed root system, thickening stems, enhancing cold resistance, drought resistance, and achieving the purpose of seedling strengthening.
Materials and Instruments
Rice Seed Move I. Materials, instrumentation and reagents 2. Spraying Analysis of results For more product details, please visit Aladdin Scientific website.
Polyazole reagent
Electronic Analytical Balance Enameled Basin Balance Ruler Small Sprayer 300 ml Beaker
1. Materials: Rice seeds.
2. Instrumentation: electronic analytical balance, 24×18 cm enamel basin, balance, straightedge, small sprayer, 300 ml beaker.
3. Reagents and preparation
There are two kinds of reagents, one is 15% wettable powder, the other is pure white crystalline product.
15% wettable polyconazole powder preparation method: weighing powder 6.67g plus distilled water to 1000 ml, stir, that is, 1000?g - ml-1 of polyconazole mother liquor, and then diluted according to the concentration required for the experiment, dilution and use of the process of attention to stirring.
Pure crystalline polyazole configuration: weigh 1g of pure product, 1g of sodium dodecyl sulfate, Tween 8 ml stir, then add 90 ml of ethanol to heat and dissolve with distilled water to 1000 ml, that is, 1000?g - ml-1 of the master batch of polyazole, and then diluted according to the concentration required for the experiment.
Experimental steps
1. Seed soaking method
1.1 Series of concentration of polychloraz solution preparation
Take 1000?g- ml-1 of the mother solution of doxorubicin and dilute it into 10, 50, 100?g- ml-1 doxorubicin solution respectively.
1.2 Seed treatment
Rice seeds were taken and soaked in water to remove the blighted grains, sterilized by 0.1% mercuric chloride immersion for about 10 min, and then rinsed several times with distilled water. The rice seeds were soaked in H2O (CK) and 10, 50, 100?g- ml-1 polyconazole solution for about 5 h. The height of the solution was appropriate to submerge the seeds.
1.3 Seedling culture
After soaking the seeds for a predetermined time, each treatment was rinsed three times with distilled water, then sown into four treatments of ceramic trays (24 × 18 cm) with appropriate amount of water, and cultivated under natural light at 25-30°C for 15-20 d (during which appropriate amount of water was frequently replenished to prevent drought).
1.4 Measurement results
Thirty plants were taken from each treatment to measure the average plant height, fresh weight of above-ground parts, fresh weight of roots, root length, and number of root strips, and the experimental results were recorded according to the following table. 
2.1 Preparation of a series of solutions of concentration of strobilurin
Take 1000?g- ml-1 of the parent solution of polyconazole, respectively diluted into 10, 100, 200?g- ml-1 polyconazole solution.
2.2 Rice seedling culture and treatment
Rice seeds were soaked in water to remove blighted grains, sterilized by 0.1% mercuric acid immersion for about 10 min, rinsed with distilled water for several times, then sown in several enameled trays (24×18 cm) and kept at a certain level of moisture, and cultivated under natural light at 25-30°C until the seedlings grew to 1 needle or 1 leaf and 1 needle with different concentrations of polyconazole treatment.
2.3 Four trays of seedlings were taken, numbered, and sprayed with H2O (CK) and 10, 100, and 200 ?g- ml-1 polyconazole solution with a sprayer until water beads hung on the surface of the seedlings, and then placed in the light to cultivate for 15-20 d (during which the appropriate amount of water was often supplemented to prevent drought).
2.4 Measurement results
After 10-15d of cultivation, 30-50 plants were taken from each treatment to measure the average plant height, fresh weight of aboveground parts, fresh weight of roots, root length and number of root strips. And the results were recorded in a table (the table was the same as the above method of seed dipping).
Based on the above results, the effect of polyazole on the growth of rice seedlings was analyzed and the significance of its application in agricultural production was discussed.