PD1/CD279 Rat mAb - Primary antibody, specific to PD1/CD279, Rat IgG2a

Cat. No.: Ab120765

GRADE & PURITY Carrier Free, Validated, Azide Free, PBS Only, ≥95%(SDS-PAGE), See COA

Application

Animal Model, Blocking, Flow Cytometry, Functional Assay, IF/ICC, IHC, Neutralization, WB

Host species

Rat

Reactive species

Mouse

Isotype

Rat IgG2a

Clone Number

29F.1A12

Conjugation

Unconjugated

Overview

Rat anti Mouse PD1/CD279 Antibody, Monoclonal (29F.1A12), could be used for in vivo blocking of PD-1/PD-L signaling, in vitro PD-1 neutralization, IHC, ICC/IF, WB, Flow and so on.
Application:
in vivo blocking of PD-1/PD-L signaling: Use at an assay dependent concentration.
in vitro PD-1 neutralization: Use at an assay dependent concentration.
IHC: Use at an assay dependent concentration.
ICC/IF: Use at an assay dependent concentration.
WB: Use at an assay dependent concentration.
Flow: Use at an assay dependent concentration.
Protein Function
Possible cell death inducer, in association with other factors.

Specifications

Product Name

PD1/CD279 Rat mAb - Primary antibody, specific to PD1/CD279, Rat IgG2a

Synonyms

Pdc1 | Ly101 | CD279 antigen | CD279 | hPD-1 | PD1 | PD-1 | PD1hPD-l | PDCD1 | programmed cell death 1 | programmed cell death protein 1 | Protein PD-1 | SLEB2 | CD279 antibody | CD279 antigen antibody | hPD 1 antibody | hPD l antibody | hPD-1 antibody |

Specifications & Purity

Carrier Free, Azide Free, Validated, PBS Only, ≥95%(SDS-PAGE), See COA

Host species

Rat

Specificity

PD1/CD279

Immunogen

Recombinant protein of mouse PD-1-Ig fusion

Conjugation

Unconjugated

Grade

Azide Free, Carrier Free, PBS Only, Validated

Isotype

Rat IgG2a

Purification Method

Protein G purified

Concentration

See COA

Storage and Shipping

Shape

Liquid

Storage

Store at 2-8°C,Avoid repeated freezing and thawing

Shipped In

Wet ice

Stability And Storage

Shipped at 4°C. Store at 4°C long term (12 months). Do not freeze. Upon receipt, it is recommended to aliquot.

Images

Flow Cytometry: PD1/CD279 Rat mAb (Ab120765)

PD1/CD279 Rat mAb (Ab120765) - Flow Cytometry
Two-color flow cytometric analysis of PD1/CD279 expression on mouse splenocytes. Flow Cytometry analysis of unstimulated (left) or 500 ng/mL Ionomycin (I139530) and 10 ng/mL PMA (P408905) co-stimulated (right) C57BL/6 mouse spleen cells were stained with CD4 Rat mAb (FITC) (Ab181110) and PD1/CD279 Rat mAb (Ab120765) at 0.5 μg/mL. Two-color flow cytometric dot plots showing the correlated expression of CD4 versus PD1/CD279 (or Isotype control staining) were derived from gated events with the forward and side light-scatter characteristics of viable mononuclear cells.

Application

• Application: Dilution info
• WB:

  Use at an assay dependent concentration.;

• IHC:

  Use at an assay dependent concentration.;

• IF/ICC:

  Use at an assay dependent concentration.;

• Flow Cytometry:

  Use at an assay dependent concentration.

• in vivo blocking of PD-1/PD-L signaling:

  Use at an assay dependent concentration.

• in vitro PD-1 neutralization:

  Use at an assay dependent concentration.;

Associated Targets(Human)

PDCD1 Tclin Programmed cell death protein 1 (0 Activities)

Discover Target: PDCD1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

PDCD1 Tclin Programmed cell death protein 1 (14 Activities)

Discover Target: PDCD1

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

Mechanisms of Action