GRADE & PURITYRecombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.See COA
Recombinant, ExactAb™, Validated, See COA ExactAb™,Recombinant,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Rabbit anti Human Bcl-2 Antibody, Recombinant, could be used for WB, IHC, IF/ICC and so on.
Specifications
Product Name
Recombinant Bcl-2 Antibody - Primary antibody, specific to Bcl-2, Rabbit IgG
Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. In
Host species
Rabbit
Specificity
Bcl-2
Immunogen
A synthetic peptide derived from human Bcl-2 (AA 48-81)
Positive Control
WB: HeLa, A375, Jurkat, MCF7, THP-1, U937, NIH/3T3 and C6 cell lysates. IHC: Rat liver, stomach, Human non-Hodgkin's lymphoma, squamous cell carcinoma, spleen, Mouse skin tissues. IF/ICC: Jurkat cells.
Conjugation
Unconjugated
Grade
ExactAb™, Recombinant, Validated
Isotype
Rabbit IgG
Light Chain Type
kappa
SDS-PAGE
150 kDa
Purification Method
Protein A purified
Concentration
See COA
Storage and Shipping
Shape
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 2-8℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images
Recombinant Bcl-2 Antibody (Ab090774) - Western Blot All lanes: Recombinant Bcl-2 Antibody (Ab090774) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 26 kDa Observed band size: 17, 26, 59 kDa Forms homodimers, and heterodimers with BAX, BAD, BAK and Bcl-X(L). Heterodimerization with BAX requires intact BH1 and BH2 motifs, and is necessary for anti-apoptotic activity (PubMed:25609812, PubMed:8183370). Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity, causes the release of cytochrome c into the cytosol promoting further caspase activity. Exposure time: 9.3 s
Recombinant Bcl-2 Antibody (Ab090774) - IHC Immunohistochemical analysis of paraffin-embedded Rat liver, using Recombinant Bcl-2 Antibody (Ab090774) at 1/100 dilution.
Recombinant Bcl-2 Antibody (Ab090774) - IHC Immunohistochemical analysis of paraffin-embedded Rat stomach, using Recombinant Bcl-2 Antibody (Ab090774) at 1/100 dilution.
Recombinant Bcl-2 Antibody (Ab090774) - IHC Immunohistochemical analysis of paraffin-embedded Human non-Hodgkin's lymphoma, using Recombinant Bcl-2 Antibody (Ab090774) at 1/100 dilution.
Recombinant Bcl-2 Antibody (Ab090774) - IHC Immunohistochemical analysis of paraffin-embedded Human squamous cell carcinoma , using Recombinant Bcl-2 Antibody (Ab090774) at 1/100 dilution.
Recombinant Bcl-2 Antibody (Ab090774) - IHC Immunohistochemical analysis of paraffin-embedded Mouse skin, using Recombinant Bcl-2 Antibody (Ab090774) at 1/100 dilution.
Recombinant Bcl-2 Antibody (Ab090774) - IHC Immunohistochemical analysis of paraffin-embedded human spleen, using Recombinant Bcl-2 Antibody (Ab090774).
Recombinant Bcl-2 Antibody (Ab090774) - IF/ICC Immunofluorescent analysis of Jurkat cells, using Recombinant Bcl-2 Antibody (Ab090774).
Application
Application
Dilution info
IHC
1/100 - 1/200
WB
1/1000 - 1/2000
IF/ICC
1/50 - 1/200
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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