Recombinant CD45 Antibody (AF405) - Primary antibody, specific to Mouse CD45

Cat. No.: Ab213882

GRADE & PURITY Validated, ExactAb™, 0.5 mg/mL

Application

Flow Cytometry

Host species

Rat

Reactive species

Mouse

Isotype

Rat IgG2b

Clone Number

P07H10

Conjugation

AF405

Overview

Recombinant CD45 Antibody, clone P07H10, could be used for Flow Cytometry.

Specifications

Product Name

Recombinant CD45 Antibody (AF405) - Primary antibody, specific to Mouse CD45

Synonyms

B220 antibody | CD 45 antibody | CD45 antibody | CD45 antigen antibody | CD45R antibody | GP180 antibody | L-CA antibody | LCA antibody | Leukocyte common antigen antibody | loc antibody | Ly-5 antibody | LY5 antibody | Ly5, homolog of antibody | Lyt-4 an

Specifications & Purity

ExactAb™, Validated, 0.5 mg/mL

Biochemical and Physiological Mechanisms

Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affec

Host species

Rat

Specificity

Mouse CD45

Immunogen

Mouse thymus or spleen

Positive Control

Flow Cytometry: C57BL/6 mouse splenocytes

Conjugation

AF405

Excitation (nm)

Violet Laser(405nm)

Emission(nm)

422nm

Ex/Em(nm)

Ex:401nm;Em:422nm

Grade

ExactAb™, Validated

Light Chain Type

kappa

Purification Method

Protein A purified

Concentration

0.5 mg/mL

Storage and Shipping

Storage

Store at 2-8°C,Protected from light,Do not freeze

Shipped In

Wet ice,Do not freeze

Stability And Storage

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Images

Flow Cytometry: Recombinant CD45 Antibody (AF405) (Ab213882)

Recombinant CD45 Antibody (AF405) (Ab213882) - Flow Cytometry
Flow cytometry analysis of CD45 expression by C57BL/6 mouse splenocytes (right panel) compared to an isotype control (left panel). Cells were stained with Recombinant CD45 Antibody (AF405) (Ab213882) at 0.3μg/test for 1 hour at 4°C. Left panel - Rat IgG (AF405) (Ab190070).

Flow Cytometry: Recombinant CD45 Antibody (AF405) (Ab213882)

Recombinant CD45 Antibody (AF405) (Ab213882) - Flow Cytometry
Flow cytometry analysis of CD45 expression by C57BL/6 mouse splenocytes (red) compared to an isotype control (blue). Cells were stained with Recombinant CD45 Antibody (AF405) (Ab213882) at 0.3μg/test for 1 hour at 4°C. Blue - Rat IgG (AF405) (Ab190070). Black - Unlabelled control, cells without incubation with primary antibody.

Application

• Application: Dilution info
• Flow Cytometry:

  ≤1.0μg/ test

  For flow cytometric staining, the suggested use of this reagent is ≤1.0 μg per million cells in 100 µL staining volume.;

Associated Targets(Human)

PTPRC Tchem Receptor-type tyrosine-protein phosphatase C (0 Activities)

Discover Target: PTPRC

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)