CD45.2 Mouse mAb - Primary antibody, specific to CD45.2, Mouse IgG2a

Cat. No.: Ab094926

GRADE & PURITY Carrier Free, Validated, ExactAb™, Azide Free, PBS Only, ≥95%(SDS-PAGE), See COA

Application

Animal Model, Blocking, Flow Cytometry, Functional Assay, IHC

Host species

Mouse

Reactive species

Mouse

Isotype

Mouse IgG2a

Clone Number

104.2

Conjugation

Unconjugated

Overview

Mouse anti Mouse CD45.2 Antibody, Monoclonal (104.2), could be used for Flow, in vivo CD45.2 blockade, in vitro CD45.2 blockade, IHC and so on.
Application:
Flow: Use at an assay dependent concentration.
in vivo CD45.2 blockade: Use at an assay dependent concentration.
in vitro CD45.2 blockade: Use at an assay dependent concentration.
IHC: Use at an assay dependent concentration.
Protein Function
Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN.

Specifications

Product Name

CD45.2 Mouse mAb - Primary antibody, specific to CD45.2, Mouse IgG2a

Synonyms

loc | Cd45 | Ly-5 | Lyt-4 | B220 | CD_antigen: CD45 | CD45 antigen | CD45R | EC 3.1.3.48 | EC:3.1.3.48 | GP180 | LCA | L-CA | Leukocyte common antigen | LY5 | protein tyrosine phosphatase, receptor type, c polypeptide | PTPRC | receptor-type tyrosine-prot

Specifications & Purity

Carrier Free, ExactAb™, Azide Free, Validated, PBS Only, ≥95%(SDS-PAGE), See COA

Host species

Mouse

Specificity

CD45.2

Immunogen

B10.S mouse thymocytes and splenocytes

Conjugation

Unconjugated

Grade

Azide Free, Carrier Free, ExactAb™, PBS Only, Validated

Isotype

Mouse IgG2a

Light Chain Type

kappa

SDS-PAGE

150 kDa

Purification Method

Protein G purified

Concentration

See COA

Storage and Shipping

Shape

Liquid

Storage

Store at 2-8°C,Do not freeze

Shipped In

Wet ice,Do not freeze

Stability And Storage

Shipped at 4°C. Store at 4°C long term (12 months). Do not freeze. Upon receipt, it is recommended to aliquot.

Images

Flow Cytometry: CD45.2 Mouse mAb (Ab094926)

CD45.2 Mouse mAb (Ab094926) - Flow Cytometry
Flow Cytometry analysis of mouse splenocytes cells labelling CD45.2 with CD45.2 Mouse mAb (Ab094926) conjugated with biotin (right panel) at 0.5 μg/mL for 1 hour at 4°C, compared with Mouse IgG Isotype Control Antibody (Biotin) (Ab180306) (left panel) and detected with SA-PE (rp156150).

Flow Cytometry: CD45.2 Mouse mAb (Ab094926)

CD45.2 Mouse mAb (Ab094926) - Flow Cytometry
Flow Cytometry analysis of mouse splenocytes cells labelling CD45.2 (red) with CD45.2 Mouse mAb (Ab094926) conjugated with biotin at 0.5 μg/mL for 1 hour at 4°C, and detected with SA-PE (rp156150). Blue - Mouse IgG Isotype Control Antibody (Biotin) (Ab180306). Black - Unlabelled control, cells without incubation with primary antibody.

Application

• Application: Dilution info
• IHC:

  Use at an assay dependent concentration.;

• Flow Cytometry:

  Use at an assay dependent concentration.

• in vivo CD45.2 blockade:

  Use at an assay dependent concentration.;

• in vitro CD45.2 blockade:

  Use at an assay dependent concentration.;