CD45 Rat mAb (Biotin) - Primary antibody, specific to CD45

Cat. No.: Ab185147

GRADE & PURITY Validated, ExactAb™, 0.5 mg/mL

Application

Flow Cytometry

Host species

Rat

Reactive species

Mouse

Isotype

Rat IgG2b

Clone Number

I3/2.3

Conjugation

Biotin

Overview

Rat anti Mouse CD45 Antibody, Monoclonal (I3/2.3), could be used for Flow and so on.

Specifications

Product Name

CD45 Rat mAb (Biotin) - Primary antibody, specific to CD45

Synonyms

B220 | CD_antigen: CD45 | CD45 antigen | CD45 | CD45R | EC 3.1.3.48 | EC:3.1.3.48 | GP180 | LCA | L-CA | Leukocyte common antigen | LY5 | protein tyrosine phosphatase, receptor type, c polypeptide | CD45 Monoclonal Antibody (I3/2.3), Biotin | Biotin anti-

Specifications & Purity

ExactAb™, Validated, 0.5 mg/mL

Host species

Rat

Specificity

CD45

Immunogen

Mouse lymphoma cell line

Positive Control

Flow: BALB/C mouse splenocytes.

Conjugation

Biotin

Grade

ExactAb™, Validated

Purification Method

Protein G purified

Concentration

0.5 mg/mL

Storage and Shipping

Storage

Store at 2-8°C,Protected from light,Do not freeze

Shipped In

Wet ice,Do not freeze

Stability And Storage

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Images

Flow Cytometry: CD45 Rat mAb (Biotin) (Ab185147)

CD45 Rat mAb (Biotin) (Ab185147) - Flow Cytometry
Flow cytometry analysis of CD45 expression by BALB/C mouse splenocytes (right panel) compared to an isotype control (left panel). Cells were stained with CD45 Rat mAb (Biotin) (Ab185147) at 0.1μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Left panel - Rat IgG (Biotin) (Ab180310).

Flow Cytometry: CD45 Rat mAb (Biotin) (Ab185147)

CD45 Rat mAb (Biotin) (Ab185147) - Flow Cytometry
Flow cytometry analysis of CD45 expression by BALB/C mouse splenocytes (red) compared to an isotype control (blue). Cells were stained with CD45 Rat mAb (Biotin) (Ab185147) at 0.1μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Blue - Rat IgG (Biotin) (Ab180310). Black - Unlabelled control, cells without incubation with primary antibody.

Application

• Application: Dilution info
• Flow Cytometry:

  ≤0.3 μg/test, the suggested use of this reagent is ≤0.3 μg per million cells in 100 µL staining volume ;

Associated Targets(Human)

PTPRC Tchem Receptor-type tyrosine-protein phosphatase C (0 Activities)

Discover Target: PTPRC

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mechanisms of Action