GRADE & PURITYRecombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.0.7 mg/mL
ExactAb™, Validated, Recombinant, 0.7 mg/mL ExactAb™,Recombinant,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Rabbit anti Human SIRP alpha Antibody, Recombinant, could be used for WB, ICC, IF and so on. Application: WB: 1/500-1/1000 ICC/IF: 1/50-1/200 Protein Function Immunoglobulin-like cell surface receptor for CD47. Acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. Supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. May play a key role in intracellular signaling during synaptogenesis and in synaptic function (By similarity). Involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin. Mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells.
Specifications
Product Name
Recombinant SIRP alpha Antibody - Primary antibody, specific to SIRPA, Rabbit IgG
Synonyms
Signal regulatory protein alpha type 1 antibody | Bit antibody | Brain Ig like molecule with tyrosine based activation motifs antibody | Brain Ig-like molecule with tyrosine-based activation motifs antibody | Brain immunoglobulin like molecule with tyrosi
Specifications & Purity
ExactAb™, Validated, Recombinant, 0.7 mg/mL
Host species
Rabbit
Specificity
SIRPA
Immunogen
Recombinant protein of human SIRP alpha (AA 31-373)
Positive Control
WB: HEK293, A549, U937 and THP-1 cell lysates. ICC/IF: HeLa cells.
Conjugation
Unconjugated
Grade
ExactAb™, Recombinant, Validated
Isotype
Rabbit IgG
SDS-PAGE
150 kDa
Purification Method
Antigen affinity purified
Concentration
0.7 mg/mL
Note
This product is nearing end-of-life and will be discontinued. The replacement product is Ab127637.
Storage and Shipping
Shape
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images
Recombinant SIRP alpha Antibody (Ab127634) - Western Blot All lanes: Recombinant SIRP alpha Antibody (Ab127634) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 55 kDa Observed band size: 74 kDa Exposure time: 65.4 s
Recombinant SIRP alpha Antibody (Ab127634) - Western Blot All lanes: Recombinant SIRP alpha Antibody (Ab127634) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 55 kDa Observed band size: 77 kDa Exposure time: 30.0 s
Recombinant SIRP alpha Antibody (Ab127634) - IF Immunocytochemistry analysis of SIRP alpha (green) in HeLa using Recombinant SIRP alpha Antibody (Ab127634), and DAPI (blue).
Application
Application
Dilution info
WB
1/500-1/1000
IF/ICC
1/50-1/200
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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