GRADE & PURITYRecombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.0.5 mg/mL
ExactAb™, Validated, Recombinant, 0.5 mg/mL ExactAb™,Recombinant,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Rabbit anti Human TRIM25 Antibody, Recombinant, could be used for WB, ICC, IF and so on. Application: WB: 1/500-1/1000 ICC/IF: 1/50-1/200 Protein Function Functions as an ubiquitin E3 ligase and as an ISG15 E3 ligase. Involved in innate immune defense against viruses by mediating ubiquitination of DDX58. Mediates 'Lys-63'-linked polyubiquitination of the DDX58 N-terminal CARD-like region which is crucial for triggering the cytosolic signal transduction that leads to the production of interferons in response to viral infection. Promotes ISGylation of 14-3-3 sigma (SFN), an adapter protein implicated in the regulation of a large spectrum signaling pathway. Mediates estrogen action in various target organs.
Specifications
Product Name
Recombinant TRIM25 Antibody - Primary antibody, specific to TRIM25, Rabbit IgG
Synonyms
2.3.2.27 | EC 6.3.2.n3 | Estrogen-responsive finger protein | RING finger protein 147 | RING-type E3 ubiquitin transferase | RING-type E3 ubiquitin transferase TRIM25 | Tripartite motif-containing protein 25 | Ubiquitin/ISG15-conjugating enzyme TRIM25 | Z
Specifications & Purity
ExactAb™, Validated, Recombinant, 0.5 mg/mL
Host species
Rabbit
Specificity
TRIM25
Immunogen
A synthetic peptide derived from human TRIM25 (AA 142-157)
Positive Control
WB: K562, HeLa and C6 cell lysates. ICC/IF: MCF7 cells.
Conjugation
Unconjugated
Grade
ExactAb™, Recombinant, Validated
Isotype
Rabbit IgG
SDS-PAGE
150 kDa
Purification Method
Antigen affinity purified
Concentration
0.5 mg/mL
Storage and Shipping
Shape
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images
Recombinant TRIM25 Antibody (Ab132484) - Western Blot All lanes: Recombinant TRIM25 Antibody (Ab132484) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 71 kDa Observed band size: 69 kDa Exposure time: 17.0 s
Recombinant TRIM25 Antibody (Ab132484) - Western Blot All lanes: Recombinant TRIM25 Antibody (Ab132484) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 71 kDa Observed band size: 69 kDa Exposure time: 1.0 s
Recombinant TRIM25 Antibody (Ab132484) - IF/ICC Immunocytochemistry analysis of TRIM25/EFP (green) in MCF7 using Recombinant TRIM25 Antibody (Ab132484), and cells were counterstained with DAPI (blue).
Application
Application
Dilution info
WB
1/500-1/1000
IF/ICC
1/50-1/200
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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