Transferrin Receptor/CD71 Rat mAb (Biotin) - Primary antibody, specific to Transferrin Receptor/CD71

Cat. No.: Ab190201

GRADE & PURITY Validated, ExactAb™, 0.5 mg/mL

Application

Flow Cytometry

Host species

Rat

Reactive species

Mouse

Isotype

Rat IgG2a

Clone Number

R17 217.1.3

Conjugation

Biotin

Overview

Rat anti Mouse Transferrin Receptor/CD71 Antibody, Monoclonal (R17 217.1.3), could be used for Flow, in vivo depletion of CD71+ cells and so on.

Specifications

Product Name

Transferrin Receptor/CD71 Rat mAb (Biotin) - Primary antibody, specific to Transferrin Receptor/CD71

Synonyms

TFR | Trfr | Mtvr1 | 2610028K12Rik | E430033M20Rik | CD71 antigen | CD71 | CD71TRFR | p90 | T9 | TfR (Transferrin R) | TfR (TransferrinR) | TFR1 | TFRC | TR | transferrin receptor (p90, CD71) | transferrin receptor protein 1 | CD 71 antibody | CD71 antibo

Specifications & Purity

ExactAb™, Validated, 0.5 mg/mL

Biochemical and Physiological Mechanisms

Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface wi

Host species

Rat

Specificity

Transferrin Receptor/CD71

Immunogen

Mouse erythroleukemia cell line 745.6

Positive Control

Flow: C57BL/6 mouse bone marrow cells

Conjugation

Biotin

Grade

ExactAb™, Validated

Light Chain Type

kappa

Purification Method

Protein G purified

Concentration

0.5 mg/mL

Storage and Shipping

Storage

Store at 2-8°C,Protected from light,Do not freeze

Shipped In

Wet ice,Do not freeze

Stability And Storage

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Images

Flow Cytometry: Transferrin Receptor/CD71 Rat mAb (Biotin) (Ab190201)

Transferrin Receptor/CD71 Rat mAb (Biotin) (Ab190201) - Flow Cytometry
Flow cytometry analysis of Transferrin Receptor/CD71 expression by C57BL/6 mouse bone marrow cells (right panel) compared to an isotype control (left panel). Cells were stained with Transferrin Receptor/CD71 Rat mAb (Biotin) (Ab190201) at 0.05μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Left panel - Rat IgG (Biotin) (Ab180310).

Flow Cytometry: Transferrin Receptor/CD71 Rat mAb (Biotin) (Ab190201)

Transferrin Receptor/CD71 Rat mAb (Biotin) (Ab190201) - Flow Cytometry
Flow cytometry analysis of Transferrin Receptor/CD71 expression by C57BL/6 mouse bone marrow cells (red) compared to an isotype control (blue). Cells were stained with Transferrin Receptor/CD71 Rat mAb (Biotin) (Ab190201) at 0.05μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Blue - Rat IgG (Biotin) (Ab180310). Black - Unlabelled control, cells without incubation with primary antibody.

Application

• Application: Dilution info
• Flow Cytometry:

  ≤0.3 μg/test, the suggested use of this reagent is ≤0.3 μg per million cells in 100 µL staining volume ;

Associated Targets(Human)

TFRC Tclin Transferrin receptor protein 1 (0 Activities)

Discover Target: TFRC

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

TFRC Tclin Transferrin receptor protein 1 (0 Activities)

Discover Target: TFRC

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

Mechanisms of Action