UltraBio™ RNA Clean Magnetic Beads

Cat. No.: R751581

Synonyms

RNA Clean Beads | RNA Cleaner | RNA Clean XP | RNA Cleanup & Concentration | NGS Cleanup Magnetic Beads | RNA Clean & Concentrator | RNA Clean-Up

Storage

Store at 2-8°C,Do not freeze

Shipped In

Wet ice,Do not freeze

Application

Nucleic Acid Extraction and Purification

Overview

UltraBio™ RNA Clean Magnetic Beads based on Solid Phase Reverse Immobilization (SPRI) technology, these beads utilize a novel nucleic acid purification medium coating combined with an optimized buffer system to purify RNA from diverse sources. They efficiently remove protein, salt ions, and other impurities, enhancing RNA purity—ideal for RNA purification in library construction workflows.

User-Supplied Reagents & Equipment

1. Reagents

80% Ethanol (prepared with Nuclease-free H₂O)

Nuclease-free H₂O

2. Equipment

Nucleic acid extraction instrument

Microcentrifuge tubes or deep-well plates

Vortex mixer

Pipettes

Thermostatic shaker

Manual Operation Protocol

1.Binding

Add 1.8× sample volume of RNA Purification Beads to the RNA solution. 

Vortex-mix thoroughly and incubate at room temperature for 5 min.

Place the tube on a magnetic rack until the solution clears completely. Discard the supernatant.

2.Wash 1

Keep the tube on the magnetic rack.

Add 200 μL 80% ethanol (prepared with Nuclease-free H₂O).

Incubate for 1 min (do not resuspend beads).

Discard the supernatant (keep tube on the rack).

3.Wash 2

Repeat Step 2. 

4.Ethanol Removal

Remove the tube from the magnetic rack.

Air-dry for 2–5 min at room temperature.

Critical: Avoid over-drying beads to maintain purification efficiency.

5.Elution

Add a user-defined volume of Nuclease-free H₂O.

Vortex-mix thoroughly and incubate at room temperature for 5 min.

6.RNA Recovery

Place the tube on the magnetic rack for 2–5 min until the solution clears. 

Transfer the supernatant to a new Nuclease-free tube.

Automated Extraction: 16/32-Channel Instrument

1. Plate Setup (96-Well Plate)

Sample Position	1, 7	2, 8	3, 9	6, 12
Reagent	Sample + 1.8× sample volume RNA Clean Magnetic Beads	200 μL 80% ethanol	200 μL 80% ethanol	Nuclease-free H₂O (volume adjusted as needed)

2. Extraction Procedure

Load the prepared 96-well plate into the instrument.

Insert magnetic tip sets.

Select and execute the instrument-specific program.

3. Post-Run Processing

Transfer eluted RNA from columns 6 and 12 to clean Nuclease-free tubes.

Automated Extraction: 96-Channel Instrument

1. Plate Setup (96-Well Plate)

Plate Position	1	2	3	6
Reagent per Well	1 Sample + 1.8× sample volume RNA Clean Magnetic Beads	200 μL 80% ethanol	200 μL 80% ethanol	Nuclease-free H₂O (volume adjusted as needed)

Note: Simultaneously processes 96 samples.

2. Extraction Procedure

Load the prepared plate into the instrument.

Insert magnetic tip sets.

Execute the instrument-specific program.

3. Post-Run Processing

Seal elution plates for immediate use or transfer RNA to Nuclease-free PCR plates.

Store at -20°C.

Precautions:

Bead Suspension Handling: This reagent is supplied as a bead suspension. DO NOT freeze or centrifuge. Vortex-mix thoroughly before use.

Nuclease-Free Practice: Use DNase/RNase-free consumables: Centrifuge tubes, Pipette tips, Microplates

Pre-Use Equilibration: Equilibrate beads to room temperature (RT) for ≥30 minutes prior to use.

Avoid Over-Drying: During ethanol removal (Step 4): Air-dry beads for ≤5 minutes. Over-drying significantly reduces RNA yield/recovery.

Specifications

Synonyms

RNA Clean Beads | RNA Cleaner | RNA Clean XP | RNA Cleanup & Concentration | NGS Cleanup Magnetic Beads | RNA Clean & Concentrator | RNA Clean-Up

Stability And Storage

Store at 2-8℃ long term (12 months). Do not freeze.

Storage

Store at 2-8°C, Do not freeze

Shipped In

Wet ice, Do not freeze

This product requires cold chain shipping. Ground and other economy services are not available.