ExactAb™, Validated, Azide Free, Ex:498nm, Em:517nm, 5μL/test Azide Free,Em:517nm,Ex:498nm,ExactAb™,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
Store at 2-8°C,Protected from light,Do not freeze Ships Wet ice,Do not freeze Check lot-specific COA for exact specifications.
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Application:
Flow: 5μL/test
For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume.
CD25 Rat mAb (FITC) (Ab179009) - Flow Cytometry
BALB/c mouse splenocytes are stained with CD25 Rat mAb (FITC) (Ab179009) and CD4 Rat mAb (APC) (Ab181112) (right). BALB/c mouse splenocytes are stained with CD4 Rat mAb (APC) (Ab181112) and Rat IgG Isotype Control Antibody (FITC) (Ab180311) (left).
CD25 Rat mAb (FITC) (Ab179009) - Flow Cytometry
Con A-stimulated (1 day) BALB/c mouse splenocytes (right panel) stained with CD25 Rat mAb (FITC) (Ab179009) (red) compared to an isotype control (blue). Blue - Rat IgG Isotype Control Antibody (FITC) (Ab180311). Black - Unlabelled control, cells without incubation with primary antibody. Untreated BALB/c mouse splenocytes were used as control (left panel).
| Application | Dilution info |
|---|---|
| Flow Cytometry | 5μl/test For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. |
Comprehensive hazard, handling, storage, and regulatory compliance document.
Download SDS →Lot-specific quality data. Enter your lot number to retrieve the exact COA.
Look up COA →Full quality attributes and acceptance criteria for this grade.
View spec sheet →| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
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| Activity Type | Relation | Activity value | Units | Action Type | Journal | PubMed Id | doi | Assay Aladdin ID |
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Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0404887 | Certificate of Analysis | Apr 25, 2024 | Ab179009 |
| ZJ24F0404886 | Certificate of Analysis | Apr 25, 2024 | Ab179009 |
| ZJ24F0404885 | Certificate of Analysis | Apr 25, 2024 | Ab179009 |
Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.
View Validated grade guide → View ExactAb™ grade guide → View Azide Free grade guide → View Ex:498nm grade guide → View Em:517nm grade guide →
