GRADE & PURITYAnimal Free?Animal-free — produced without animal-derived components to reduce contamination risk. Use in biomanufacturing and culture avoiding animal-origin material.Carrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Bioactive?Bioactive grade — verified to retain biological activity in functional assays. Use when the molecule must be functionally active, not just pure.High Performance?High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short.His Tag?His tag grade — recombinant protein bearing a His tag for affinity purification/detection. Use to purify, immobilize, or detect the tagged protein.PBS Only?PBS-only formulation — supplied in phosphate-buffered saline with no other additives. Use when you need a clean PBS buffer free of stabilizers/carriers.≥95%(SDS-PAGE)
Expression System
HEK293
Accession #
O14763
Protein Tag
C-His
Expression system
HEK293
Endotoxin Concentration
<1.0 EU/μg
Bioactivity
Recombinant Human TRAIL R2/TNFRSF10B Protein (rp176693) inhibits Recombinant Human TRAIL mediated cytotoxicity using L-929 mouse fibroblast cells. The ED50 for this effect is 27.85 ng/mL. Immobilized Recombinant Human TRAIL R2/TNFRSF10B Protein (rp176693) at 5.0 μg/mL can bind Recombinant Human TRAIL Protein (rp169692) with the EC50 of 41.55 ng/mL.
Animal Free,Carrier Free,Bioactive,High Performance,His Tag,PBS Only,≥95%(SDS-PAGE) Animal Free,Bioactive,Carrier Free,High Performance,His Tag,PBS Only for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Purity: ≥95%, by SDS-PAGE visualized with Coomassie® Blue Staining. Description： Tumor necrosis factor receptor superfamily, member 10b, official symbol TNFRSF10B, also known as Death receptor 5, CD262, TNF-related apoptosis-inducing ligand receptor 2 (TRAIL R2), is a member of the TNF-receptor superfamily, and contains an intracellular death domain. This receptor can be activated by tumor necrosis factor-related apoptosis inducing ligand (TNFSF10/TRAIL/APO-2L), and transduces an apoptosis signal. Studies with FADD-deficient mice suggested that FADD, a death domain containing adaptor protein, is required for the apoptosis mediated by this protein. TRAIL R2/CD262/TNFRSF10B was purified independently as the only receptor for TRAIL detectable on the surface of two different human cell lines that undergo apoptosis upon stimulation with TRAIL. TRAIL R2/CD262/TNFRSF10B contains two extracellular cysteine-rich repeats, typical for TNF receptor (TNFR) family members, and a cytoplasmic death domain. TRAIL R2/CD262/TNFRSF10B mediates apoptosis via the intracellular adaptor molecule FADD/MORT1. TRAIL receptors can signal both death and gene transcription, functions reminiscent of those of TNFR1 and TRAMP, two other members of the death receptor family. Defects in TRAIL R2/CD262/TNFRSF10B may be a cause of head and neck squamous cell carcinomas (HNSCC) also known as squamous cell carcinoma of the head and neck.
Specifications
Product Name
Recombinant Human TRAIL R2/TNFRSF10B Protein
Synonyms
CD262 antigen | CD262 | death domain containing receptor for TRAIL/Apo-2L | Death receptor 5 | DR5 | DR5TRICK2B | Fas-like protein | KILLER/DR5 | KILLERapoptosis inducing protein TRICK2A/2B | TNF-related apoptosis-inducing ligand receptor 2 | TNFRSF10B |
Grade
Animal Free, Bioactive, Carrier Free, High Performance, His Tag, PBS Only
Specifications & Purity
Animal Free, Carrier Free, Bioactive, High Performance, His Tag, PBS Only, ≥95%(SDS-PAGE)
Bioactivity
Recombinant Human TRAIL R2/TNFRSF10B Protein (rp176693) inhibits Recombinant Human TRAIL mediated cytotoxicity using L-929 mouse fibroblast cells. The ED50 for this effect is 27.85 ng/mL. Immobilized Recombinant Human TRAIL R2/TNFRSF10B Protein (rp176693) at 5.0 μg/mL can bind Recombinant Human TRAIL Protein (rp169692) with the EC50 of 41.55 ng/mL.
14.7&18.7 kDa, under reducing conditions; 13.7&17.5 kDa, under non-reducing conditions
Molecule Type
Protein
Storage and Shipping
Shape
Lyophilized
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 0.5 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at -20°C for 1 year. Avoid freeze / thaw cycle.
Images
Recombinant Human TRAIL R2/TNFRSF10B Protein (rp176693)- Protein Bioactivity
Recombinant Human TRAIL R2/TNFRSF10B Protein (rp176693) inhibits Recombinant Human TRAIL mediated cytotoxicity using L-929 mouse fibroblast cells. The ED₅₀ for this effect is 27.85 ng/mL.
Recombinant Human TRAIL R2/TNFRSF10B Protein (rp176693) - ELISA Immobilized Recombinant Human TRAIL R2/TNFRSF10B Protein (rp176693) at 5.0 μg/mL can bind Recombinant Human TRAIL Protein (rp169692) with the EC₅₀ of 41.55 ng/mL.
Recombinant Human TRAIL R2/TNFRSF10B Protein (rp176693) - SDS-PAGE 3 μg/lane of Recombinant Human TRAIL R2/TNFRSF10B Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 14.7&18.7 kDa under reducing conditions and 13.7&17.5 kDa under non-reducing conditions.
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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