Tricine - for cell culture, ≥99.5%(T), used for Tricine serves as a versatile buffering agent suitable for separating low molecular weight proteins in lower percent acrylamide gels, has demonstrated efficacy in ATP assays employing firefly luciferase, and

CAS: 5704-04-1 Cat. No.: T118067 Molecular Weight: 179.17 Beilstein Registry Number: 1937804 EC Number: 227-193-6 PubChem CID: 79784

GRADE & PURITY for Cell culture, ≥99.5%(T)

Synonyms

2-(1,3-dihydroxy-2-(hydroxymethyl)propan-2-ylamino)acetic acid | C6H13NO5 | 2-{[1,3-dihydroxy-2-(hydroxymethyl)propan-2-yl]amino}acetic acid | N-[2-hydroxy-1,1-bis(hydroxymethyl)ethyl]glycine | AKOS015856413 | 2,6,6-Trimethyl-2-cyclohexen-1-carboxaldehyde

Storage

Room temperature

Shipped In

Normal

Overview

Tricine is a zwitterionic biological buffer commonly used in enzyme assays, electrophoresis, and cell culture research. It has a higher negative charge compared to glycine, which enables it to migrate more rapidly. Tricine′s high ionic strength has the ability to cause more ion movement and less protein movement, resulting in the separation of low molecular weight proteins in lower percent acrylamide gels. It has been observed that Tricine is efficient in the separation of proteins ranging from 1 to 100 kDa through electrophoresis. According to a study, the 25 mmol/L Tricine buffer proved to be the most effective among ten other buffers tested for ATP assays using firefly luciferase. Further, Tricine is also used in capillary zone electrophoresis, high performance liquid chromatography and ion exchange chromatography.

Specifications

Synonyms

2-(1, 3-dihydroxy-2-(hydroxymethyl)propan-2-ylamino)acetic acid | C6H13NO5 | 2-{[1, 3-dihydroxy-2-(hydroxymethyl)propan-2-yl]amino}acetic acid | N-[2-hydroxy-1, 1-bis(hydroxymethyl)ethyl]glycine | AKOS015856413 | 2, 6, 6-Trimethyl-2-cyclohexen-1-carboxaldehyde

Specifications & Purity

for cell culture, ≥99.5%(T)

Application

Tricine serves as a versatile buffering agent suitable for separating low molecular weight proteins in lower percent acrylamide gels, has demonstrated efficacy in ATP assays employing firefly luciferase, and has shown potential in scavenging hydroxyl radi

Storage

Room temperature

Shipped In

Normal

Grade

for Cell culture

Purity

≥99.5%(T)

Names and Identifiers

• Pubchem Sid: 504755419
• Pubchem Sid Url: https://pubchem.ncbi.nlm.nih.gov/substance/504755419
• Canonical Smiles: C(C(=O)O)NC(CO)(CO)CO
• IUPAC Name: 2-[[1,3-dihydroxy-2-(hydroxymethyl)propan-2-yl]amino]acetic acid
• InChIKey: SEQKRHFRPICQDD-UHFFFAOYSA-N
• INCHI: 1S/C6H13NO5/c8-2-6(3-9,4-10)7-1-5(11)12/h7-10H,1-4H2,(H,11,12)
• Isomeric SMILES: C(C(=O)O)NC(CO)(CO)CO
• WGK Germany: 3
• PubChem CID: 79784
• Molecular Weight: 179.17
• Beilstein: 1937804
• Reaxy-Rn: 1937804

Taxonomic Classification

Taxonomy Tree

• Kingdom Organic compounds
  • Superclass Organic acids and derivatives
    • Class Carboxylic acids and derivatives
      • Subclass Amino acids, peptides, and analogues
        • Level5 Amino acids and derivatives
          • Level6 Alpha amino acids and derivatives
            • Level7 Alpha amino acids

• Kingdom: Organic compounds
• Superclass: Organic acids and derivatives
• Class: Carboxylic acids and derivatives
• Subclass: Amino acids, peptides, and analogues
• Intermediate Tree Nodes: Amino acids and derivatives - Alpha amino acids and derivatives
• Direct Parent: Alpha amino acids
• Alternative Parents: Amino acids 1,2-aminoalcohols Monocarboxylic acids and derivatives Dialkylamines Carboxylic acids Primary alcohols Organopnictogen compounds Organic oxides Hydrocarbon derivatives Carbonyl compounds
• Molecular Framework: Aliphatic acyclic compounds
• Substituents: Alpha-amino acid - 1,2-aminoalcohol - Amino acid - Carboxylic acid - Secondary aliphatic amine - Monocarboxylic acid or derivatives - Secondary amine - Organic nitrogen compound - Primary alcohol - Organooxygen compound - Organonitrogen compound - Hydrocarbon derivative - Organic oxide - Organopnictogen compound - Carbonyl group - Amine - Organic oxygen compound - Alcohol - Aliphatic acyclic compound
• Description: This compound belongs to the class of organic compounds known as alpha amino acids. These are amino acids in which the amino group is attached to the carbon atom immediately adjacent to the carboxylate group (alpha carbon).
• External Descriptors: tricine

Data sources

1. Djoumbou Feunang Y, Eisner R, Knox C, Chepelev L, Hastings J, Owen G, Fahy E, Steinbeck C, Subramanian S, Bolton E, Greiner R, and Wishart DS. ClassyFire: Automated Chemical Classification With A Comprehensive, Computable Taxonomy. Journal of Cheminformatics, 2016, 8:61.

DOI: 10.1186/s13321-016-0174-y

Chemical and Physical Properties

• Solubility: Soluble in water (89.6 g/l) at 20 °C.
• Melt Point(°C): 172 °C
• Molecular Weight: 179.170 g/mol
• XLogP3: -5.100
• Hydrogen Bond Donor Count: 5
• Hydrogen Bond Acceptor Count: 6
• Rotatable Bond Count: 6
• Exact Mass: 179.079 Da
• Monoisotopic Mass: 179.079 Da
• Topological Polar Surface Area: 110.000 Ų
• Heavy Atom Count: 12
• Formal Charge: 0
• Complexity: 137.000
• Isotope Atom Count: 0
• Defined Atom Stereocenter Count: 0
• Undefined Atom Stereocenter Count: 0
• Defined Bond Stereocenter Count: 0
• Undefined Bond Stereocenter Count: 0
• The total count of all stereochemical bonds: 0
• Covalently-Bonded Unit Count: 1

References

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