CD11b Rat mAb (Biotin) - Primary antibody, specific to CD11b, Rat IgG2b

Cat. No.: Ab179521

GRADE & PURITY Validated, ExactAb™, 0.5 mg/mL

Application

Flow Cytometry

Host species

Rat

Reactive species

Human, Mouse

Isotype

Rat IgG2b

Clone Number

M1/70

Conjugation

Biotin

Overview

Application:
Flow: ≤0.1 μg/test
For flow cytometric staining, the suggested use of this reagent is ≤0.1 μg per million cells in 100 µL staining volume.

Specifications

Product Name

CD11b Rat mAb (Biotin) - Primary antibody, specific to CD11b, Rat IgG2b

Synonyms

antigen CD11b (p170) | CD11 antigen-like family member B | CD11b antigen | CD11b | Cell surface glycoprotein MAC-1 subunit alpha | Complement component 3 receptor 3 subunit | CR-3 alpha chain | CR3A | CR3AMGC117044 | Integrin alpha M | integrin alpha-M |

Specifications & Purity

ExactAb™, Validated, 0.5 mg/mL

Host species

Rat

Specificity

CD11b

Immunogen

C57BL/10 splenocytes

Positive Control

Flow: Human peripheral blood monocyte

Conjugation

Biotin

Grade

ExactAb™, Validated

Isotype

Rat IgG2b

Purification Method

Protein G purified

Concentration

0.5 mg/mL

Storage and Shipping

Storage

Store at 2-8°C,Protected from light,Do not freeze

Shipped In

Wet ice,Do not freeze

Stability And Storage

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Images

Flow Cytometry: CD11b Rat mAb (Biotin) (Ab179521)

CD11b Rat mAb (Biotin) (Ab179521) - Flow Cytometry
Flow cytometry analysis of CD11b expression by Human peripheral blood (right panel) compared to an isotype control (left panel). Cells were stained with CD11b Rat mAb (Biotin) (Ab179521) at 0.01μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Left panel – Rat IgG Isotype Control Antibody (Biotin) (Ab180310).

Flow Cytometry: CD11b Rat mAb (Biotin) (Ab179521)

CD11b Rat mAb (Biotin) (Ab179521) - Flow Cytometry
Flow cytometry analysis of CD11b expression by Human peripheral blood monocytes (red) compared to an isotype control (blue). Cells were stained with CD11b Rat mAb (Biotin) (Ab179521) at 0.01μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Blue - Rat IgG Isotype Control Antibody (Biotin) (Ab180310). Black - Unlabelled control, cells without incubation with primary antibody.

Application

• Application: Dilution info
• Flow Cytometry:

  ≤0.1 μg/test

  For flow cytometric staining, the suggested use of this reagent is ≤0.1 μg per million cells in 100 µL staining volume.;

Associated Targets(Human)

ITGAM Tbio Integrin alpha-M (0 Activities)

Discover Target: ITGAM

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mechanisms of Action