CLEC9A/CD370 Rat mAb - Primary antibody, specific to CLEC9A/CD370, Rat IgG1

Cat. No.: Ab096837

GRADE & PURITY Carrier Free, Validated, Azide Free, PBS Only, ≥95%(SDS-PAGE), See COA

Application

ELISA, Flow Cytometry, IF/ICC, IP, WB

Host species

Rat

Reactive species

Mouse

Isotype

Rat IgG1

Clone Number

7H11

Conjugation

Unconjugated

Overview

Rat anti Mouse CLEC9A/CD370 Antibody, Monoclonal (7H11), could be used for in vivo CLEC9A blockade, in vivo Ag targeting to CLEC9A+ DCs, WB, ELISA, IP, IF/ICC, Flow and so on.
Application
in vivo CLEC9A blockade: Use at an assay dependent concentration.
in vivo Ag targeting to CLEC9A+ DCs: Use at an assay dependent concentration.
WB: Use at an assay dependent concentration.
ELISA: Use at an assay dependent concentration.
IP: Use at an assay dependent concentration.
IF/ICC: Use at an assay dependent concentration.
Flow: Use at an assay dependent concentration.
Protein Function
Functions as an endocytic receptor on a small subset of myeloid cells specialized for the uptake and processing of material from dead cells. Recognizes filamentous form of actin in association with particular actin-binding domains of cytoskeletal proteins, including spectrin, exposed when cell membranes are damaged, and mediate the cross-presentation of dead-cell associated antigens in a Syk-dependent manner.

Specifications

Product Name

CLEC9A/CD370 Rat mAb - Primary antibody, specific to CLEC9A/CD370, Rat IgG1

Synonyms

DNGR-1 | 9830005G06Rik | CD370 | CLEC9a | C-type lectin domain family 9 member A | C-type lectin domain family 9, member A | DNGR1 | HEEE9341 | UNQ9341 | C type lectin domain family 9, member A antibody | C-type lectin domain family 9 member A antibody |

Specifications & Purity

Carrier Free, Azide Free, Validated, PBS Only, ≥95%(SDS-PAGE), See COA

Host species

Rat

Specificity

CLEC9A/CD370

Immunogen

RBL-2H3 cells expressing mouse CLEC9A fused to an HA epitope

Conjugation

Unconjugated

Grade

Azide Free, Carrier Free, PBS Only, Validated

Isotype

Rat IgG1

Light Chain Type

kappa

SDS-PAGE

150 kDa

Purification Method

Protein G purified

Concentration

See COA

Storage and Shipping

Shape

Liquid

Storage

Store at 2-8°C,Do not freeze

Shipped In

Wet ice,Do not freeze

Stability And Storage

Shipped at 4°C. Store at 4°C long term (12 months). Do not freeze. Upon receipt, it is recommended to aliquot.

Images

Flow Cytometry: CLEC9A/CD370 Rat mAb (Ab096837)

CLEC9A/CD370 Rat mAb (Ab096837) - Flow Cytometry
Multicolor flow cytometric analysis of CLEC9A/CD370 expression on live mouse splenocytes. C57BL/6 mouse splenocytes were stained with CD11c Armenian Hamster mAb (APC) (Ab178872), CD8 alpha Rat mAb (FITC) (Ab190208) and either Rat IgG Isotype Control Antibody (Biotin) (Ab180310; Left panel) or CLEC9A/CD370 Rat mAb (Ab096837; Right panel) at 1.0 μg/mL. A bivariate pseudocolor density plot showing the correlated expression of CLEC9A/CD370 expression (or Isotype control staining) was derived from CD8+ gated events with the forward and side light-scatter characteristics of viable splenocytes.

Application

• Application: Dilution info
• WB:

  Use at an assay dependent concentration.;

• IP:

  Use at an assay dependent concentration.

• Flow Cytometry:

  Use at an assay dependent concentration.

• ELISA:

  Use at an assay dependent concentration.;

• IF/ICC:

  Use at an assay dependent concentration.;

Associated Targets(Human)

CLEC9A Tbio C-type lectin domain family 9 member A (0 Activities)

Discover Target: CLEC9A

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)