Collagenase NB 4 From Clostridium Histolyticum, CAS No.9001-12-1

CAS: 9001-12-1 Cat. No.: C279013 EC Number: 232-582-9 PubChem CID: 44384985

GRADE & PURITY EnzymoPure™, Standard Grade,≥ 0.10 U/mg

Overview

Collagenase NB 4 is a crude collagenase that contains collagenolytic and additional enzymatic activities including clostripain and neutral protease. The balanced ratio of these activities ensures gentle and efficient tissue dissociation. 

Application

Collagenase NB 4 is suitable for dissociation of a broad variety of tissue types.

Specifications

Contains class I and class II collagenase (1) and a balanced ratio of proteolytic side activities.

Activity (Wünsch): ≥ 0.10 U/mg

Suitable for different applications in tissue dissociation such as isolation of human and rodent hepatocytes, chondrocytes, neuronal and endothelial cells, fibroblasts, cells from adipose tissue (e.g. ADSCs), stem cells and rodent osteoblasts.

(Clostridiopeptidase A)

EC 3.4.24.3 • Mr ca. 70 000 - 120 000 (collagenases) • CAS [9001-12-1]

References and Definitions

Unit definition: 1 U according to Wünsch (2) catalyzes the hydrolysis of 1 µmole 4-phenylazobenzyloxycarbonyl-L-prolyl-L-leucylglycyl-L-prolyl-D-arginine per minute at 25 °C, pH 7.1.

References

1. Bond, M.D. & van Wart, H.E. (1984) Biochemistry 23, 3077-3091

2. Wünsch, E. & Heidrich, H.G. (1963) Hoppe-Seyler's Z. Physiol. Chem. 333, 149-51

Specifications

Product Name

Collagenase NB 4 From Clostridium Histolyticum, CAS No.9001-12-1

Synonyms

Collagenase I | Collagenase Type II | (5S, 6S, 9R, 10S, 13S, 17S, 23S, 24S, 27R, 28S, 31S, 35S)-5, 6, 9, 13, 17, 23, 24, 27, 31, 35-Decamethyl-10, 28-dioctyl-2, 20-diazanonacyclo[19.15.0.03, 19.05, 17.06, 14.09, 13.023, 35.024, 32.027, 31]hexatriaconta-1(21), 2, 19-triene

Grade

EnzymoPure™

Specifications & Purity

EnzymoPure™, Standard Grade, ≥ 0.10 U/mg

CAS

9001-12-1

Enzyme Commission Number

3.4.24.3

Molecule Type

Small molecule

Storage and Shipping

Concentration

Standard Grade,≥ 0.10 U/mg

Storage

Store at 2-8°C

Shipped In

Wet ice

Unit definition

1 U according to Wünsch (2) catalyzes the hydrolysis of 1 µmole 4-phenylazobenzyloxycarbonyl-L-prolyl-L-leucylglycyl-L-prolyl-D-arginine per minute at 25 °C, pH 7.1.

Associated Targets(Human)

HT-29 (80576 Activities)

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Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

MCF7 (126967 Activities)

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Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

SK-MEL-5 (47095 Activities)

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A549 (127892 Activities)

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Mechanisms of Action

References

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