Connexin 43/GJA1 (phospho S368) Antibody - Primary antibody, specific to GJA1 (phospho S368), Rabbit IgG

Cat. No.: Ab097298

GRADE & PURITY Validated, 1.0 mg/mL

Application

IHC, WB

Host species

Rabbit

Reactive species

Human, Mouse, Rat

Isotype

Rabbit IgG

Conjugation

Unconjugated

Overview

Rabbit polyclonal to Connexin 43/GJA1 (phospho S368), could be used for WB, IHC and so on.
Applications:
WB: 1/500 - 1/2000
IHC: 1/100 - 1/300
Protein Function
One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. May play a critical role in the physiology of hearing by participating in the recycling of potassium to the cochlear endolymph.

Specifications

Product Name

Connexin 43/GJA1 (phospho S368) Antibody - Primary antibody, specific to GJA1 (phospho S368), Rabbit IgG

Synonyms

Connexin 43(phospho-Ser368) | Connexin 43(phospho Ser368) | Connexin 43(phospho S368) | Connexin 43 | Connexin43v Cx 43v CX43 | DFNB38 | Gap junction 43 kDa heart protein | Connexin-43 | Gap junction alpha 1 protein | Gap junction protein alpha 1 43kDa (c

Specifications & Purity

Validated, 1.0 mg/mL

Host species

Rabbit

Specificity

GJA1 (phospho S368)

Immunogen

A synthetic peptide derived from human Connexin 43/GJA1 (phospho S368) (AA 332-381).

Positive Control

WB: K562 treated with 200ng/mL PMA for 10 min lysates. IHC: Human breast carcinoma tissue.

Conjugation

Unconjugated

Grade

Validated

Isotype

Rabbit IgG

SDS-PAGE

150kDa

Purification Method

Immunogen affinity purified

Concentration

1.0 mg/mL

Storage and Shipping

Shape

Liquid

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at 4°C short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Images

Western Blot: Connexin 43/GJA1 (phospho S368) Antibody (Ab097298)

Connexin 43/GJA1 (phospho S368) Antibody (Ab097298) - Western Blot
All lanes: Connexin 43/GJA1 (phospho S368) Antibody (Ab097298) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/10000 dilution
Predicted band size: 43 kDa
Observed band size: 31, 37 kDa
The truncated isoforms are detected as distinct bands on the western blot lysate from the left ventricle of non-failing human heart tissue . In addition to 43 kDa full length isoform, the largest 3 isoforms (32 kDa, 29 kDa and 26 kDa in size) contain 2 transmembrane domains, part of intracellular loop, and the C-terminus. The smallest 3 isoforms are 20 kDa, 11 kDa, and 7 kDa in size and include part of the C-terminal tail with the 20 kDa isoform also containing part of the last transmembrane domain (PMID: 28576298).
Exposure time: 26.2 s

IHC: Connexin 43/GJA1 (phospho S368) Antibody (Ab097298)

Connexin 43/GJA1 (phospho S368) Antibody (Ab097298) - IHC
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Connexin 43/GJA1 (phospho S368) Antibody (Ab097298). The picture on the right is blocked with the phosphor peptide.

Application

• Application: Dilution info
• WB:

  1/500-1/2000;

• IHC:

  1/100-1/300

Associated Targets(Human)

GJA1 Tbio Gap junction alpha-1 protein (0 Activities)

Discover Target: GJA1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mechanisms of Action