Goat Anti-Human IgG (Biotin) - Secondary antibody

Cat. No.: Ab175832
AVAILABLE TO ORDER
GRADE & PURITY Validated ? Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated. ExactAb™ ? ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications. Azide Free ? Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory. High Performance ? High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short. 1.0 mg/mL
Application
ELISA, Flow Cytometry, IF/ICC, WB
Host species
Goat
Reactive species
Human
Conjugation
Biotin
 ·  off list, applied to all prices below.
Size
Status
Price
Qty
100μg
Ab175832-100μg
10
$31.90
1mg
Ab175832-1mg
1-2 wks(?)
Item is derived from our semi-finished stock and is processed in 1-2 weeks.
$239.90
Enter a quantity for the sizes you want to add.
🧪

Why this grade

ExactAb™, High Performance, Validated, Azide Free, 1.0 mg/mL Azide Free,ExactAb™,High Performance,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

🌡

Storage & shipping

Store at 2-8°C,Protected from light,Avoid repeated freezing and thawing Ships Wet ice Check lot-specific COA for exact specifications.

📋

Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

📚

Literature proof

Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

Overview

Application
ELISA: 1:10000-1:200000
WB: 1:10000-1:200000
Flow: 1:200-1:1000
IF: 1:1000

Specifications

Product Name
Goat Anti-Human IgG (Biotin) - Secondary antibody
Synonyms
Goat Anti-Human Antibody (Biotin) | Biotin-conjugated Goat Anti-Human Antibody | Goat Anti-Human IgG / Biotin antibody | Goat Anti-Human IgG-Biotin antibody
Specifications & Purity
ExactAb™, High Performance, Validated, Azide Free, 1.0 mg/mL
Host species
Goat
Conjugation
Biotin
Grade
Azide Free, ExactAb™, High Performance, Validated
Purification Method
Antigen affinity purified
Concentration
1.0 mg/mL
Storage and Shipping
Shape
Liquid
Storage
Store at 2-8°C,Protected from light,Avoid repeated freezing and thawing
Shipped In
Wet ice
Stability And Storage
Store at 4°C for one year. Avoid repeated freeze/thaw cycles. Protect from light.
Images

Goat Anti-Human IgG (Biotin) (Ab175832) - ELISA
Goat Anti-Human IgG (Biotin) (Ab175832) was tested by direct ELISA. The plate was coated with different amounts of Human IgG (Ab170213). A serial dilution of Goat Anti-Human IgG (Biotin) (Ab175832) was used as the secondary antibody. A HRP-streptavidin (np156148) was used at 1/20000 dilution for this ELISA.

Goat Anti-Human IgG (Biotin) (Ab175832) - ELISA
Cross-reactivity of Goat Anti-Human IgG (Biotin) (Ab175832) was tested by direct ELISA. The wells were coated with the indicated IgG at 0.5 µg/mL (100 µL/well) and incubated overnight at 4℃, followed by a 2% BSA blocking step for 2h. Goat Anti-Human IgG (Biotin) (Ab175832) at 1/10000 dilution was used as the secondary antibody. A HRP-streptavidin (np156148) was used at 1/20000 dilution for this ELISA.

Application
ApplicationDilution info
WB

1/10000-1/200000

IF/ICC

1:1000

Flow Cytometry

1/200 - 1/1000

ELISA

1:10000-1:200000

Documentation

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

Certificates(CoA,COO,BSE/TSE and Analysis Chart)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

4 results found

Lot NumberCertificate TypeDateItem
ZJ23F1001382Certificate of AnalysisApr 09, 2026 Ab175832
ZJ26F0433761Certificate of AnalysisApr 02, 2026 Ab175832
ZJ24R1201654Certificate of AnalysisDec 26, 2024 Ab175832
ZJ23F1001381Certificate of AnalysisOct 26, 2023 Ab175832
Documents & Articles
Solution Calculators
Reviews

Customer Reviews

Need help choosing the grade?

Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.

View Validated grade guide → View ExactAb™ grade guide → View Azide Free grade guide → View High Performance grade guide →

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