GRADE & PURITYCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.1.0 mg/mL
ExactAb™, Validated, Carrier Free, 1.0 mg/mL Carrier Free,ExactAb™,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Rabbit anti Human GPR 174 Antibody, Polyclonal, could be used for WB, IF, ICC and so on. Application: WB: 1/500-1/1000 IF/ICC: 1/100-1/500 Protein Function Putative receptor for purines coupled to G-proteins.
Specifications
Product Name
GPR 174 Antibody - Primary antibody, specific to GPR174, Rabbit IgG
Specifications & Purity
ExactAb™, Validated, Carrier Free, 1.0 mg/mL
Host species
Rabbit
Specificity
GPR174
Immunogen
A KLH-conjugated synthetic peptide encompassing a sequence within the center region of human GPR174.
Positive Control
WB: A549, Jurkat and C6 cell lysates. IF/ICC: LOVO cells.
Conjugation
Unconjugated
Grade
Carrier Free, ExactAb™, Validated
Isotype
Rabbit IgG
SDS-PAGE
150 kDa
Purification Method
Immunogen affinity purified
Concentration
1.0 mg/mL
Storage and Shipping
Shape
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images
GPR 174 Antibody (Ab106230) - Western Blot All lanes: GPR 174 Antibody (Ab106230) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/10000 dilution Predicted band size: 39 kDa Observed band size: 41 kDa Exposure time: 100.0 s
GPR 174 Antibody (Ab106230) - IF/ICC Immunofluorescent analysis of GPR 174 staining in LOVO cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with GPR 174 Antibody (Ab106230) in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with AF647-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Application
Application
Dilution info
IF/ICC
1/100-1/500
WB
1/500-1/1000
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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