Iscalimab (anti-CD40) - Anticorpo primario, specific to CD40, Human IgG1, Antibody of CD40, Antibody of CD40

CAS: 2031153-61-2 Cat. No.: Ab175491

GRADE & PURITY Animal Free, Carrier Free, Recombinant, Validated, ExactAb™, Low Endotoxin, Azide Free, ≥95%(SDS-PAGE&SEC-HPLC)

Application

Animal Model, ELISA, Flow Cytometry, Functional Assay

Host species

Umano

Reactive species

Cynomolgus monkey, Scimmia Rhesus, Umano

Isotype

Human IgG1

Conjugation

Unconjugated

Panoramica

Iscalimab (anti-CD40) is a non-depleting IGg1 monoclonal antibody targeting CD40 (KD: 0.3 nM). Iscalimab (anti-CD40) can be used for research of Graves' hyperthyroidism and autoimmune diseases.
Purity
≥95% (SDS-PAGE&SEC)
Endotoxin Level
< 1.0EU/mg

Specifications

Product Name

Iscalimab (anti-CD40) - Anticorpo primario, specific to CD40, Human IgG1, Antibody of CD40

Sinonimi

AI326936 antibody | B cell associated molecule CD40 antibody | B cell surface antigen CD40 antibody | B cell-associated molecule antibody | B-cell surface antigen CD40 antibody | Bp50 antibody | CD 40 antibody | CD40 antibody | CD40 antigen (TNF receptor

Specifiche e purezza

Carrier Free, Recombinant, ExactAb™, Low Endotoxin, Azide Free, Validated, Animal Free, ≥95%(SDS-PAGE&SEC-HPLC)

Specie ospite

Umano

Specificità

CD40

Coniugazione

Unconjugated

Grado

Animal Free, Azide Free, Carrier Free, ExactAb™, Low Endotoxin, Recombinant, Validated

Tipo di azione

INHIBITOR

Meccanismo d'azione

Antibody of CD40

Isotipo

Human IgG1

Tipo di catena leggera

kappa

SDS-PAGE

27.2 kDa (Light Chain) & 52.2 kDa (Heavy Chain), under reducing conditions; 201.2 kDa, under non-reducing conditions.

Metodo di purificazione

Protein A purified

CAS

2031153-61-2

Stoccaggio e spedizione

Forma

Liquid

Condizioni di conservazione di stoccaggio

Store at -80°C,Avoid repeated freezing and thawing

Spedito in

Dry ice packs + Cold packs

Stabilità e conservazione

Store at -80℃ for 24 months. Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Immagini

Flow Cytometry: Iscalimab (anti-CD40) (Ab175491)

Iscalimab (anti-CD40) (Ab175491) - Flow Cytometry
Flow Cytometry analysis of human peripheral blood lymphocytes labelling CD40 with Iscalimab (anti-CD40) (Ab175491) conjugated with biotin (right panel) compared with Human IgG (Biotin) (Ab180314) - Isotype Control (left panel) and detected with SA-PE (rp156150). 

ELISA: Iscalimab (anti-CD40) (Ab175491)

Iscalimab (anti-CD40) (Ab175491) - ELISA
Immobilized Recombinant Human CD40 protein (rp183577) at 1.0 μg/mL can bind Iscalimab (anti-CD40) (Ab175491) with the EC₅₀ of 35.88 ng/mL.

SEC: Iscalimab (anti-CD40) (Ab175491)

Iscalimab (anti-CD40) (Ab175491) - SEC
The purity of Iscalimab (anti-CD40) (Ab175491) is more than 95% verified by HPLC.

Flow Cytometry: Iscalimab (anti-CD40) (Ab175491)

Iscalimab (anti-CD40) (Ab175491) - Flow Cytometry
Flow Cytometry analysis of Raji cells labelling CD40 (red) with Iscalimab (anti-CD40) (Ab175491). Goat Anti-Human IgG (PE) (Ab175838) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, human IgG (Ab170213). Black - Unlabelled control, cells without incubation with primary antibody.

Flow Cytometry: Iscalimab (anti-CD40) (Ab175491)

Iscalimab (anti-CD40) (Ab175491) - Flow Cytometry
Flow Cytometry analysis of human peripheral blood lymphocytes labelling CD40 (red) with Iscalimab (anti-CD40) (Ab175491) conjugated with biotin and detected with SA-PE (rp156150). Blue - Isotype control, Human IgG (Biotin) (Ab180314). Black - Unlabelled control, cells without incubation with primary antibody.

Obiettivi associati (umani)

CD40 Tchem Tumor necrosis factor receptor superfamily member 5 (0 Activities)

Discover Target: CD40

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

CD40 Tchem Tumor necrosis factor receptor superfamily member 5 (9 Activities)

Discover Target: CD40

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

Meccanismi d'azione

Domande frequenti

What does Animal Free mean?

Animal Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?80 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships frozen with dry ice and cold packs. Unpack on arrival and transfer it to the storage condition stated above; handle dry ice with insulated gloves in a ventilated area.

What are the CAS number, molecular formula and molecular weight?

The CAS Number is 2031153-61-2.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Iscalimab (anti-CD40)); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.