MAdCAM1 Rat mAb - Primary antibody, specific to MAdCAM1, Rat IgG2a

Cat. No.: Ab113964

GRADE & PURITY Carrier Free, Validated, Azide Free, PBS Only, ≥95%(SDS-PAGE), See COA

Application

Animal Model, Blocking, Flow Cytometry, Functional Assay, IF/ICC, Neutralization

Host species

Rat

Reactive species

Mouse

Isotype

Rat IgG2a

Clone Number

MECA-367

Conjugation

Unconjugated

Overview

Rat anti Mouse MAdCAM1 Antibody, Monoclonal (MECA-367), could be used for in vivo MAdCAM-1 neutralization, IF, ICC, Flow and so on.
Application:
in vivo MAdCAM-1 neutralization: Use at an assay dependent concentration. 
ICC/IF: Use at an assay dependent concentration.
Flow: Use at an assay dependent concentration. 
Protein Function
Cell adhesion leukocyte receptor expressed by mucosal venules, helps to direct lymphocyte traffic into mucosal tissues including the Peyer patches and the intestinal lamina propria. It can bind both integrin alpha-4/beta-7 and L-selectin, regulating both the passage and retention of leukocytes. Isoform 2, lacking the mucin-like domain, may be specialized in supporting integrin alpha-4/beta-7-dependent adhesion strengthening, independent of L-selectin binding.

Specifications

Product Name

MAdCAM1 Rat mAb - Primary antibody, specific to MAdCAM1, Rat IgG2a

Synonyms

hMAdCAM-1 | MACAM1 | MAdCAM1 | MAdCAM-1 | mucosal addressin cell adhesion molecule 1 | mucosal addressin cell adhesion molecule-1 | mucosal vascular addressin cell adhesion molecule 1 | Addressin mucosal antibody | hMAdCAM 1 antibody | hMAdCAM-1 antibody

Specifications & Purity

Carrier Free, Azide Free, Validated, PBS Only, ≥95%(SDS-PAGE), See COA

Host species

Rat

Specificity

MAdCAM1

Immunogen

Endothelial cells isolated from BALB/c mouse

Conjugation

Unconjugated

Grade

Azide Free, Carrier Free, PBS Only, Validated

Isotype

Rat IgG2a

Light Chain Type

kappa

SDS-PAGE

150 kDa

Purification Method

Protein G purified

Concentration

See COA

Storage and Shipping

Shape

Liquid

Storage

Store at 2-8°C,Do not freeze

Shipped In

Wet ice,Do not freeze

Stability And Storage

Shipped at 4°C. Store at 4°C long term (12 months). Do not freeze. Upon receipt, it is recommended to aliquot.

Images

Flow Cytometry: MAdCAM1 Rat mAb (Ab113964)

MAdCAM1 Rat mAb (Ab113964) - Flow Cytometry
Flow Cytometry analysis of unstimulated (left) or 20 ng/mL TNF-alpha (rp154759) stimulated bEND3​ cells (24 h) labelling MAdCAM1 (red) with MAdCAM1 Rat mAb (Ab113964) at 10.0 μg/mL for 1 hour at 4°C conjugated with biotin and detected with SA-PE (rp156150). Blue - Isotype control, Rat IgG (Biotin) (Ab180310). Black - Unlabelled control, cells without incubation with primary antibody.

Application

• Application: Dilution info
• IF/ICC:

  Use at an assay dependent concentration.;

• Flow Cytometry:

  Use at an assay dependent concentration.

• in vivo MAdCAM-1 neutralization:

  Use at an assay dependent concentration.;

• in vivo FasL blockade:

  Use at an assay dependent concentration.;

Associated Targets(Human)

MADCAM1 Tbio Mucosal addressin cell adhesion molecule 1 (0 Activities)

Discover Target: MADCAM1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

COG1 Tbio Conserved oligomeric Golgi complex subunit 1 (0 Activities)

Discover Target: COG1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

MADCAM1 Tbio Mucosal addressin cell adhesion molecule-1 (5 Activities)

Discover Target: MADCAM1

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

Mechanisms of Action