GRADE & PURITYAnimal Free?Animal-free — produced without animal-derived components to reduce contamination risk. Use in biomanufacturing and culture avoiding animal-origin material.Carrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Bioactive?Bioactive grade — verified to retain biological activity in functional assays. Use when the molecule must be functionally active, not just pure.ActiBioPure™?ActiBioPure™ — Aladdin's premier line for bioactive and recombinant products. Use when both high purity and preserved biological activity are required.High Performance?High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short.His Tag?His tag grade — recombinant protein bearing a His tag for affinity purification/detection. Use to purify, immobilize, or detect the tagged protein.PBS Only?PBS-only formulation — supplied in phosphate-buffered saline with no other additives. Use when you need a clean PBS buffer free of stabilizers/carriers.≥95%(SDS-PAGE)
Expression System
HEK293
Accession #
P14210
Protein Tag
C-His
Expression system
HEK293
Endotoxin Concentration
<1.0 EU/μg
Bioactivity
1. Determined by the dose-dependent stimulation of the proliferation of monkey 4MBr-5 cells. The ED50 for this effect is 24.77 ng/mL. 2. Immobilized Recombinant Human HGFR/c-MET Protein (rp169642) at 1.0 μg/mL can bind Biotinylated Recombinant Human HGF Protein (rp175908) with the EC50 for this effect is 97.73 ng/mL.
ActiBioPure™, Animal Free, Bioactive, Carrier Free, High Performance, His Tag, PBS Only
Specifications & Purity
Animal Free, Carrier Free, Bioactive, ActiBioPure™, High Performance, His Tag, PBS Only, ≥95%(SDS-PAGE)
Biochemical and Physiological Mechanisms
HGF, also known as scatter factor and hepatopoietin A, is a pleiotropic protein in the plasminogen subfamily of S1 peptidases. It's a multidomain molecule with an N-terminal PAN/APPLE-like domain, four Kringle domains, and a serine proteinase-like domain
Bioactivity
1. Determined by the dose-dependent stimulation of the proliferation of monkey 4MBr-5 cells. The ED50 for this effect is 24.77 ng/mL. 2. Immobilized Recombinant Human HGFR/c-MET Protein (rp169642) at 1.0 μg/mL can bind Biotinylated Recombinant Human HGF Protein (rp175908) with the EC50 for this effect is 97.73 ng/mL.
30.0-40.0 kDa & 56.0-65.0 kDa & 85.0-95.0 kDa, under reducing condition; 67.0-77.2 kDa, under non-reducing condition
Molecule Type
Protein
Storage and Shipping
Shape
Lyophilized
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 0.5 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at -20°C stable up to 1 year. Avoid freeze / thaw cycle.
Images
Recombinant Human HGF Protein (rp175908) - Protein Bioactivity Determined by the dose-dependent stimulation of the proliferation of monkey 4MBr-5 cells. The ED
₅₀
for this effect is 24.77 ng/mL.
Recombinant Human HGF Protein (rp175908) - Protein Bioactivity Immobilized Recombinant Human HGFR/c-MET Protein (rp169642) at 1.0 μg/mL can bind Biotinylated Recombinant Human HGF Protein (rp175908) with the EC
₅₀
for this effect is 97.73 ng/mL.
Recombinant Human HGF Protein (rp175908) - ELISA Immobilized Recombinant Human HGF Protein (rp156180) at 1.0 μg/mL can bind Ficlatuzumab (anti-HGF) (Ab170563) with the EC
₅₀
of 45.64 ng/mL.
Recombinant Human HGF Protein (rp175908) - ELISA Immobilized Recombinant Human HGF Protein (rp156180) at 1.0 μg/mL can bind Rilotumumab (anti-HGF) (Ab170950) with the EC
₅₀
of 21.92 ng/mL.
Recombinant Human HGF Protein (rp175908) - SDS-PAGE 2 μg/lane of Recombinant Human HGF Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 30.0-40.0 kDa & 56.0-65.0 kDa & 85.0-95.0 kDa under reducing conditions and 67.0-77.2 kDa under non-reducing conditions.
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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