Recombinant, ExactAb™, Validated, High Performance, See COA ExactAb™,High Performance,Recombinant,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Rabbit anti Human MEK1/MEK2 Antibody, Recombinant, could be used for WB, IHC, ICC, IF and so on.
Application:
WB: 1/5000-1/10000
IHC: 1/100-1/500
ICC/IF: 1/50-1/100
Protein Function：
Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Binding of extracellular ligands such as growth factors, cytokines and hormones to their cell-surface receptors activates RAS and this initiates RAF1 activation. RAF1 then further activates the dual-specificity protein kinases MAP2K1/MEK1 and MAP2K2/MEK2. Both MAP2K1/MEK1 and MAP2K2/MEK2 function specifically in the MAPK/ERK cascade, and catalyze the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in the extracellular signal-regulated kinases MAPK3/ERK1 and MAPK1/ERK2, leading to their activation and further transduction of the signal within the MAPK/ERK cascade. Depending on the cellular context, this pathway mediates diverse biological functions such as cell growth, adhesion, survival and differentiation, predominantly through the regulation of transcription, metabolism and cytoskeletal rearrangements. One target of the MAPK/ERK cascade is peroxisome proliferator-activated receptor gamma (PPARG), a nuclear receptor that promotes differentiation and apoptosis. MAP2K1/MEK1 has been shown to export PPARG from the nucleus. The MAPK/ERK cascade is also involved in the regulation of endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC), as well as in the fragmentation of the Golgi apparatus during mitosis.
Recombinant MEK1/MEK2 Antibody (Ab114851) - Western Blot
All lanes: Recombinant MEK1/MEK2 Antibody (Ab114851) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 44 kDa
Observed band size: 42 kDa
Exposure time: 1.1 s
Recombinant MEK1/MEK2 Antibody (Ab114851) - Western Blot
All lanes use Recombinant MEK1/MEK2 Antibody (Ab114851) at 1/5000 dilution for 1 hour at room temperature.
Recombinant MEK1/MEK2 Antibody (Ab114851) - IF/ICC
Immunofluorescent analysis of HeLa cells, using Recombinant MEK1/MEK2 Antibody (Ab114851) at 1/50 dilution.
Recombinant MEK1/MEK2 Antibody (Ab114851) - IF/ICC
Immunofluorescent analysis of HeLa cells, using Recombinant MEK1/MEK2 Antibody (Ab114851) at 1/150 dilution.
Recombinant MEK1/MEK2 Antibody (Ab114851) - IF/ICC
Immunofluorescent analysis of HeLa cells, using Recombinant MEK1/MEK2 Antibody (Ab114851) at 1/200 dilution.
Recombinant MEK1/MEK2 Antibody (Ab114851) - IF/ICC
Immunofluorescent analysis of HeLa cells, using Recombinant MEK1/MEK2 Antibody (Ab114851).
Recombinant MEK1/MEK2 Antibody (Ab114851) - IHC
Immunohistochemical analysis of paraffin-embedded human kidney, using Recombinant MEK1/MEK2 Antibody (Ab114851).
| Application | Dilution info |
|---|---|
| IHC | 1/100-1/500 |
| WB |
1/5000 – 1/10000
|
| IF/ICC | 1/50-1/100 |
Comprehensive hazard, handling, storage, and regulatory compliance document.
Download SDS →Lot-specific quality data. Enter your lot number to retrieve the exact COA.
Look up COA →Full quality attributes and acceptance criteria for this grade.
View spec sheet →| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Activity Type | Relation | Activity value | Units | Action Type | Journal | PubMed Id | doi | Assay Aladdin ID |
|---|
| Activity Type | Relation | Activity value | Units | Action Type | Journal | PubMed Id | doi | Assay Aladdin ID |
|---|
| Activity Type | Relation | Activity value | Units | Action Type | Journal | PubMed Id | doi | Assay Aladdin ID |
|---|
| Activity Type | Relation | Activity value | Units | Action Type | Journal | PubMed Id | doi | Assay Aladdin ID |
|---|
| Activity Type | Relation | Activity value | Units | Action Type | Journal | PubMed Id | doi | Assay Aladdin ID |
|---|
| Activity Type | Relation | Activity value | Units | Action Type | Journal | PubMed Id | doi | Assay Aladdin ID |
|---|
| Activity Type | Relation | Activity value | Units | Action Type | Journal | PubMed Id | doi | Assay Aladdin ID |
|---|
| Activity Type | Relation | Activity value | Units | Action Type | Journal | PubMed Id | doi | Assay Aladdin ID |
|---|
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0910849 | Certificate of Analysis | Jul 10, 2026 | Ab114851 |
Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.
View Recombinant grade guide → View Validated grade guide → View ExactAb™ grade guide → View High Performance grade guide →
