Recombinant Taq DNA Polymerase Protein used for PCR various applications, CAS No.9012-90-2

CAS: 9012-90-2 Cat. No.: A156186 Molecular Weight: 94 kDa EC Number: 232-741-2

GRADE & PURITY EnzymoPure™, His Tag, ≥95%, See COA

Expression System

E. coli

Accession #

P19821

Protein Tag

N-His

Expression system

E. coli

Application

PCR

Bioactivity

Taq DNA polymerase is a heat-resistant enzyme that synthesizes DNA from a single-stranded template in the presence of dNTP and primers, with 5´→3´ DNA polymerase activity and 5´→3´ exonuclease activity.

Overview

Purity:

>95%, by SDS-PAGE visualized with Coomassie® Blue Staining.

Taq DNA Polymerase is a thermostable enzyme that synthesizes DNA from single-stranded templates in the presence of dNTPs and a primer. The enzyme consists of a single polypeptide with a molecular weight of 94 kDa. It has a 5´→3´ DNA polymerase activity and a 5´→3´ exonuclease activity.

Source：
Recombinant enzyme is purified from the cloned Thermus aquaticus DNA polymerase gene expressed in E. coli.

Unit definition

One unit of Taq DNA Polymerase is the amount of enzyme required to incorporate 10 nmoles of deoxyribonucleotide into DNA in 30 min at 74°C.

Product content：

1. Taq DNA Polymerase ( 5 U/μL )

2. 10×Taq Buffer（Mg2+Plus）：200mM Tris-HCl,500mM KCl,20mM MgCl2,1mM DTT,pH9.3

PCR Reaction Setup：

Component	50-μL rxn
5×Taq Buffer(Mg2+ Plus)	10μL
dNTP Mixture(2.5mM each)	4μL
Template DNA	＜500 ng
Primer F	0.2-1.0 μM
Primer R	0.2-1.0 μM
Taq DNA Polymerase(5U/μL)	0.2μL
ddH2O	Up to 50μL

Incubate reactions in a thermal cycler：

Step	Temperature（℃）	Time	Cycles
Initial Denaturation	95	5min	1
Denature	95	30s	25-35
Anneal	~55(depending on primer Tm)	30s
Extend	72	1min/kb
Final Extension	72	10min	1
Hold	4	indefinitely	1

Optimization Strategies：

Different PCR reaction conditions, including temperature, time and number of cycles, should be set according to different template, primer, length of PCR product and GC content.

The time setting of STEP4(extension) should be set according to the length of PCR products, usually the extension time of each kb product is 1min.

For initial PCR, the number of cycles can be set to 35 to ensure that the expected PCR product can be amplified as much as possible. The number of PCR reaction cycles that need to be semi-quantitative or quantitative must be properly optimized so that the PCR reaction does not reach a plateau.

Specifications

Product Name

Recombinant Taq DNA Polymerase Protein used for PCR various applications, CAS No.9012-90-2

Synonyms

DNA polymerase I | thermostable | DNA-directed DNA polymerase

Grade

His Tag, EnzymoPure™

Specifications & Purity

EnzymoPure™, His-Tag, ≥95%, See COA

Bioactivity

Taq DNA polymerase is a heat-resistant enzyme that synthesizes DNA from a single-stranded template in the presence of dNTP and primers, with 5´→3´ DNA polymerase activity and 5´→3´ exonuclease activity.

Expression System

E. coli

Species

Thermus aquaticus

Amino Acids

1-832 aa

Sequence

MRGMLPLFEPKGRVLLVDGHHLAYRTFHALKGLTTSRGEPVQAVYGFAKSLLKALKEDGDAVIVVFDAKAPSFRHEAYGGYKAGRAPTPEDFPRQLALIKELVDLLGLARLEVPGYEADDVLASLAKKAEKEGYEVRILTADKDLYQLLSDRIHVLHPEGYLITPAWLWEKYGLRPDQWADYRALTGDESDNLPGVKGIGEKTARKLLEEWGSLEALLKNLDRLKPAIREKILAHMDDLKLSWDLAKVRTDLPLEVDFAKRREPDRERLRAFLERLEFGSLLHEFGLLESPKALEEAPWPPPEGAFVGFVLSRKEPMWADLLALAAARGGRVHRAPEPYKALRDLKEARGLLAKDLSVLALREGLGLPPGDDPMLLAYLLDPSNTTPEGVARRYGGEWTEEAGERAALSERLFANLWGRLEGEERLLWLYREVERPLSAVLAHMEATGVRLDVAYLRALSLEVAEEIARLEAEVFRLAGHPFNLNSRDQLERVLFDELGLPAIGKTEKTGKRSTSAAVLEALREAHPIVEKILQYRELTKLKSTYIDPLPDLIHPRTGRLHTRFNQTATATGRLSSSDPNLQNIPVRTPLGQRIRRAFIAEEGWLLVALDYSQIELRVLAHLSGDENLIRVFQEGRDIHTETASWMFGVPREAVDPLMRRAAKTINFGVLYGMSAHRLSQELAIPYEEAQAFIERYFQSFPKVRAWIEKTLEEGRRRGYVETLFGRRRYVPDLEARVKSVREAAERMAFNMPVQGTAADLMKLAMVKLFPRLEEMGARMLLQVHDELVLEAPKERAEAVARLAKEVMEGVYPLAVPLEVEVGIGEDWLSAKE

Protein Tag

N-His

Protein Length

Protein fragments

Accession #

P19821

Source

Recombinant

Predicted molecular weight

96 kDa

SDS-PAGE

96 kDa

CAS

9012-90-2

Molecule Type

Protein

Storage and Shipping

Application

PCR

Shape

Liquid

Concentration

See COA

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at -20°C stable for 1 year. Avoid freeze / thaw cycle.

Images

PCR: Recombinant Taq DNA Polymerase Protein (A156186)

Recombinant Taq DNA Polymerase Protein (A156186) - PCR
Taq polymerase catalyzes oligonucleotide primer-driven, DNA template dependent incorporation of dNTPs into complimentary DNA strands. It displays both 5′ to 3′ polymerase and exonuclease activities. Use the measurements below to prepare your PCR experiment, or enter your own parameters in the column provided.

Component	50 μL rxn
5×Taq Buffer (Mg2+ Plus)	10 μL
dNTP Mixture (2.5 mM each)	4 μL
Template DNA	5 ng
Primer F	0.5 μM
Primer R	0.5 μM
Taq DNA Polymerase (5 U/μL)	0.1 μL/0.2 μL
ddH2O	Up to 50 μL

Reaction condition: 95 ℃ 3 min; 95 ℃ 10 s, 55 ℃ 30 s, 72 ℃ 1 min, 30 cycle; 72 ℃ 5 min.