The tip of the germinating wheat embryonic root was cut (about 1 cm from the tip) and immersed in neutral red solution for live staining for 10-15 min, then removed and rinsed with tap water for 5-10 min, and the epidermis was torn (or made a longitudinal section of the severed root) and examined under a microscope.
Operation method
Experimental morphological observations on vesicles in young wheat roots
Materials and Instruments
Wheat germ root Microscope Carrier plate Cover plate Tweezers Dropper blade 0.03% neutral red solution Small petri dish Move I. Materials and equipment For more product details, please visit Aladdin Scientific website.
Sprouted wheat radicle, microscope, carrier sheet, cover sheet, forceps, dropper, razor blade, 0.03% neutral red solution, small petri dish.
II. Experimental steps
Cut the tip of sprouted wheat radicle (about 1cm from the tip), immerse it in neutral red solution for 10-15 minutes, then remove it and rinse it with tap water for 5-10 minutes, tear the epidermis (or make a longitudinal section of the root) and examine it under the microscope. It can be seen: in the meristematic zone embryonic cells there are a small number of sporadic small red dots, indicating that the vesicle has begun to form; into the elongation of the cell there is a red reticulated interlacing vesicle structure; in the mature section of the cell, there is a very complete large vesicle, generally located in the center of the cell.