1.0 mg/mL for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Rabbit anti Human NIFK Antibody, Polyclonal, could be used for WB, IHC, IF, ICC and so on. Application: WB: 1/500-1/2000 IHC: 1/100-1/300 IF/ICC: 1/200-1/1000 Post-translational Sequentially phosphorylated on Thr-238, Thr-234 and Ser-230. Thr-234 is phosphorylated only when Thr-238 is phosphorylated. Likewise, phosphorylation at Ser-230 requires that Thr-234 and Thr-238 are phosphorylated. Phosphorylation enhances MKI67 binding.
Specifications
Product Name
NIFK Antibody - Primary antibody, specific to NIFK, Rabbit IgG
A synthetic peptide derived from human NIFK (AA 200-249)
Epitope
Binds to an epitope located within the peptidesequence IDYDFPSLILQKTES as determined by overlapping synthetic peptides.
Positive Control
WB: HeLa, NIH/3T3 and C6 cell lysates. IHC: Human brain tissue. IF/ICC: HUVEC cells.
Conjugation
Unconjugated
Isotype
Rabbit IgG
SDS-PAGE
150 kDa
Purification Method
Immunogen affinity purified
Concentration
1.0 mg/mL
Storage and Shipping
Shape
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images
NIFK Antibody (Ab118085) - Western Blot All lanes: NIFK Antibody (Ab118085) at 1/500 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/5000 dilution Predicted band size: 34 kDa Observed band size: 31 kDa Exposure time: 150.0 s
NIFK Antibody (Ab118085) - IHC Immunohistochemistry analysis of paraffin-embedded human brain tissue, using NIFK Antibody (Ab118085). The picture on the right is blocked with the synthesized peptide.
NIFK Antibody (Ab118085) - IF/ICC Immunofluorescence analysis of HUVEC cells, using NIFK Antibody (Ab118085). The picture on the right is blocked with the synthesized peptide.
Application
Application
Dilution info
WB
1/500-1/2000
IHC
1/100-1/300
IF/ICC
1/200-1/1000
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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