GRADE & PURITYRecombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.0.2 mg/mL
Protein Function Adapter protein which associates with tyrosine-phosphorylated growth factor receptors or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. Plays a role in the DNA damage response, not in the detection of the damage by ATM/ATR, but for efficient activation of downstream effectors, such as that of CHEK2.
Specifications
Product Name
Recombinant Nck Antibody - Primary antibody, specific to NCK1, Rabbit IgG
Synonyms
cytoplasmic protein NCK1 | MGC12668 | NCK adaptor protein 1melanoma NCK protein | NCK1 | nck-1 | NCKalpha | NCKNCK tyrosine kinase | non-catalytic region of tyrosine kinase | SH2/SH3 adaptor protein NCK-alpha | Cytoplasmic protein NCK1 antibody | MGC12668
Specifications & Purity
ExactAb™, Validated, Recombinant, 0.2 mg/mL
Host species
Rabbit
Specificity
NCK1
Immunogen
A synthetic peptide derived from human Nck (AA 248-266)
Positive Control
WB: K562, HeLa, HEK293, A431, Jurkat, NIH/3T3 and C6 cell lysates. IHC: Human lung cancer tissue. IF/ICC: HeLa cells.
Conjugation
Unconjugated
Grade
ExactAb™, Recombinant, Validated
Isotype
Rabbit IgG
Light Chain Type
kappa
SDS-PAGE
150 kDa
Purification Method
Antigen affinity purified
Concentration
0.2 mg/mL
Storage and Shipping
Shape
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images
Recombinant Nck Antibody (Ab117238) - Western Blot All lanes: Recombinant Nck Antibody (Ab117238) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 43 kDa Observed band size: 46 kDa Exposure time: 1.1 s
Recombinant Nck Antibody (Ab117238) - IF/ICC Immunocytochemistry analysis of NCK1 (green) in HeLa using Recombinant Nck Antibody (Ab117238), and cells were counterstained with DAPI (blue).
Recombinant Nck Antibody (Ab117238) - IHC Immunohistochemistry analysis of paraffin-embedded Human lung cancer tissue using Recombinant Nck Antibody (Ab117238). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Application
Application
Dilution info
WB
1/500-1/1000
IHC
1/50 - 1/100
IF/ICC
1/50 - 1/200
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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