GRADE & PURITY Carrier Free, Validated, ExactAb™, Azide Free, PBS Only, ≥95%(SDS-PAGE), See COA

Application

Animal Model, Blocking, Flow Cytometry, Functional Assay, IP, WB

Host species

Rat

Reactive species

Mouse

Isotype

Rat IgG1

Clone Number

P84

Conjugation

Unconjugated

Overview

Rat anti Mouse SIRP alpha/CD172a Antibody, Monoclonal (P84), could be used for in vivo SIRPα blocking, in vitro SIRPα blocking, WB, IP, Flow and so on.

Application:

in vivo SIRPα blocking: Use at an assay dependent concentration. 

in vitro SIRPα blocking: Use at an assay dependent concentration. 

WB: Use at an assay dependent concentration. 

IP: Use at an assay dependent concentration. 

Flow: Use at an assay dependent concentration. 
Protein Function
Immunoglobulin-like cell surface receptor for CD47. Acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. Supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. May play a key role in intracellular signaling during synaptogenesis and in synaptic function (By similarity). Involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin. Mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells.

Specifications

Product Name

SIRP alpha/CD172a Rat mAb - Primary antibody, specific to SIRP alpha/CD172a, Rat IgG1

Synonyms

Bit | SIRP | SHP-1 | Ptpns1 | Idd13.2 | BIT | BITbrain-immunoglobulin-like molecule with tyrosine-based activation motifs | Brain Ig-like molecule with tyrosine-based activation motifs | CD172 antigen-like family member A | CD172a antigen | CD172a | Inhib

Specifications & Purity

Carrier Free, ExactAb™, Azide Free, Validated, PBS Only, ≥95%(SDS-PAGE), See COA

Host species

Rat

Specificity

SIRP alpha/CD172a

Immunogen

Mouse brain membrane protein

Conjugation

Unconjugated

Grade

Azide Free, Carrier Free, ExactAb™, PBS Only, Validated

Isotype

Rat IgG1

Light Chain Type

kappa

SDS-PAGE

150 kDa

Purification Method

Protein A purified

Concentration

See COA

Storage and Shipping

Shape

Liquid

Storage

Store at 2-8°C,Do not freeze

Shipped In

Wet ice,Do not freeze

Stability And Storage

Shipped at 4°C. Store at 4°C long term (12 months). Do not freeze. Upon receipt, it is recommended to aliquot.

Images

Flow Cytometry: SIRP alpha/CD172a Rat mAb (Ab127636)

SIRP alpha/CD172a Rat mAb (Ab127636) - Flow Cytometry
Two-color flow cytometric analysis of SIRP alpha/CD172a expression on mouse bone marrow cells. C57BL/6 mouse bone marrow cells were stained with CD11b Rat mAb (APC) (Ab178881) and either Rat IgG Isotype Control Antibody (Biotin) (Ab180310; Left panel) or SIRP alpha/CD172a Rat mAb (Ab127636; Right panel) at 0.5 μg/mL. Two-color flow cytometric dot plots showing the correlated expression of CD11b versus SIRP alpha/CD172a (or Isotype control staining) were derived from gated events with the forward and side light-scatter characteristics of viable mononuclear cells.

Application

• Application: Dilution info
• WB:

  Use at an assay dependent concentration.;

• IP:

  Use at an assay dependent concentration.

• Flow Cytometry:

  Use at an assay dependent concentration.

• in vivo SIRPα blocking:

  Use at an assay dependent concentration;

• in vitro SIRPα blocking:

  Use at an assay dependent concentration;

Associated Targets(Human)

SIRPA Tbio Tyrosine-protein phosphatase non-receptor type substrate 1 (0 Activities)

Discover Target: SIRPA

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)