X-Gal (5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside) - Ultra pure , CAS No.7240-90-6

CAS: 7240-90-6 Cat. No.: X274331 Molecular Weight: 408.63 Beilstein Registry Number: 1402009 EC Number: 230-640-8

GRADE & PURITY Ultra pure

Synonyms

(2S,3R,4S,5R,6R)-2-[(5-bromo-4-chloro-1H-indol-3-yl)oxy]-6-(hydroxymethyl)tetrahydropyran-3,4,5-triol | A-D-galactopyranoside | GALACTOPYRANOSIDE, 5-BROMO-4-CHLOROINDOL-3-YL, .BETA.-D- | 5-Bromo-4-chloro-3-indolyl beta-D-galactopyranoside, Vetec(TM) reage

Storage

Store at 2-8°C

Shipped In

Wet ice

Specifications

Synonyms

(2S, 3R, 4S, 5R, 6R)-2-[(5-bromo-4-chloro-1H-indol-3-yl)oxy]-6-(hydroxymethyl)tetrahydropyran-3, 4, 5-triol | A-D-galactopyranoside | GALACTOPYRANOSIDE, 5-BROMO-4-CHLOROINDOL-3-YL, .BETA.-D- | 5-Bromo-4-chloro-3-indolyl beta-D-galactopyranoside, Vetec(TM) reage

Specifications & Purity

Ultra pure

Storage

Store at 2-8°C

Shipped In

Wet ice

This product requires cold chain shipping. Ground and other economy services are not available.

Grade

Ultra pure

Names and Identifiers

• Pubchem Sid: 504753967
• Pubchem Sid Url: https://pubchem.ncbi.nlm.nih.gov/substance/504753967
• Canonical Smiles: C1=CC(=C(C2=C1NC=C2OC3C(C(C(C(O3)CO)O)O)O)Cl)Br
• IUPAC Name: (2S,3R,4S,5R,6R)-2-[(5-bromo-4-chloro-1H-indol-3-yl)oxy]-6-(hydroxymethyl)oxane-3,4,5-triol
• InChIKey: OPIFSICVWOWJMJ-AEOCFKNESA-N
• INCHI: 1S/C14H15BrClNO6/c15-5-1-2-6-9(10(5)16)7(3-17-6)22-14-13(21)12(20)11(19)8(4-18)23-14/h1-3,8,11-14,17-21H,4H2/t8-,11+,12+,13-,14-/m1/s1
• Isomeric SMILES: C1=CC(=C(C2=C1NC=C2O[C@H]3[C@@H]([C@H]([C@H]([C@H](O3)CO)O)O)O)Cl)Br
• WGK Germany: 3
• Molecular Weight: 408.63
• Beilstein: 1402009
• Reaxy-Rn: 32869881
• Reaxys-RN_link_address: https://www.reaxys.com/reaxys/secured/hopinto.do?context=S&query=IDE.XRN=32869881&ln=

Taxonomic Classification

Taxonomy Tree

• Kingdom Organic compounds
  • Superclass Organic oxygen compounds
    • Class Organooxygen compounds
      • Subclass Carbohydrates and carbohydrate conjugates
        • Level5 Glycosyl compounds
          • Level6 O-glycosyl compounds

• Kingdom: Organic compounds
• Superclass: Organic oxygen compounds
• Class: Organooxygen compounds
• Subclass: Carbohydrates and carbohydrate conjugates
• Intermediate Tree Nodes: Glycosyl compounds
• Direct Parent: O-glycosyl compounds
• Alternative Parents: Hexoses Indoles Substituted pyrroles Aryl bromides Aryl chlorides Oxanes Benzenoids Heteroaromatic compounds Secondary alcohols Acetals Polyols Azacyclic compounds Oxacyclic compounds Organopnictogen compounds Primary alcohols Organonitrogen compounds Hydrocarbon derivatives Organochlorides Organobromides
• Molecular Framework: Aromatic heteropolycyclic compounds
• Substituents: Hexose monosaccharide - O-glycosyl compound - Indole - Indole or derivatives - Aryl bromide - Aryl chloride - Aryl halide - Monosaccharide - Oxane - Substituted pyrrole - Benzenoid - Pyrrole - Heteroaromatic compound - Secondary alcohol - Oxacycle - Polyol - Azacycle - Organoheterocyclic compound - Acetal - Primary alcohol - Organohalogen compound - Hydrocarbon derivative - Organopnictogen compound - Organobromide - Organochloride - Organic nitrogen compound - Organonitrogen compound - Alcohol - Aromatic heteropolycyclic compound
• Description: This compound belongs to the class of organic compounds known as o-glycosyl compounds. These are glycoside in which a sugar group is bonded through one carbon to another group via a O-glycosidic bond.
• External Descriptors: organobromine compound - organochlorine compound - beta-D-galactoside - indolyl carbohydrate - D-aldohexose derivative

Data sources

1. Djoumbou Feunang Y, Eisner R, Knox C, Chepelev L, Hastings J, Owen G, Fahy E, Steinbeck C, Subramanian S, Bolton E, Greiner R, and Wishart DS. ClassyFire: Automated Chemical Classification With A Comprehensive, Computable Taxonomy. Journal of Cheminformatics, 2016, 8:61.

DOI: 10.1186/s13321-016-0174-y

Mechanisms of Action

Chemical and Physical Properties

• Specific Rotation[α]: -66 ° (C=1, DMF)
• Melt Point(°C): 236°C
• Molecular Weight: 408.630 g/mol
• XLogP3: 1.200
• Hydrogen Bond Donor Count: 5
• Hydrogen Bond Acceptor Count: 6
• Rotatable Bond Count: 3
• Exact Mass: 406.977 Da
• Monoisotopic Mass: 406.977 Da
• Topological Polar Surface Area: 115.000 Ų
• Heavy Atom Count: 23
• Formal Charge: 0
• Complexity: 421.000
• Isotope Atom Count: 0
• Defined Atom Stereocenter Count: 5
• Undefined Atom Stereocenter Count: 0
• Defined Bond Stereocenter Count: 0
• Undefined Bond Stereocenter Count: 0
• The total count of all stereochemical bonds: 0
• Covalently-Bonded Unit Count: 1

References

1. Chunxu Chen, Tianhui Li, Guijie Chen, Dan Chen, Yujia Peng, Bing Hu, Yi Sun, Xiaoxiong Zeng.  (2020)  Commensal Relationship of Three Bifidobacterial Species Leads to Increase of Bifidobacterium in Vitro Fermentation of Sialylated Immunoglobulin G by Human Gut Microbiota.  JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,      [PMID:32806107] [10.1021/acs.jafc.0c03628]

2. Zhi-P. Cai, Louis P. Conway, Ying Y. Huang, Wen J. Wang, Pedro Laborda, Ting Wang, Ai M. Lu, Hong L. Yao, Kun Huang, Sabine L. Flitsch, Li Liu, Josef Voglmeir.  (2019)  Enzymatic Synthesis of Trideuterated Sialosides.  MOLECULES,  24  (7): (1368).  [PMID:30965582] [10.3390/molecules24071368]

3. Xiaochong Zhu, Jieyuan Wu, Shizhong Li, La Xiang, Jian-Ming Jin, Chaoning Liang, Shuang-Yan Tang.  (2024)  Artificial Biosynthetic Pathway for Efficient Synthesis of Vanillin, a Feruloyl-CoA-Derived Natural Product from Eugenol.  JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,      [PMID:38501643] [10.1021/acs.jafc.3c08723]

4. Zi-Xuan Hu, Shu-Rui Li, Qing-Jun Xia, Ting Wang, Josef Voglmeir, Göran Widmalm, Li Liu.  (2024)  Enzymatic synthesis of N-formylated sialosides via a five-enzyme cascade.  ORGANIC & BIOMOLECULAR CHEMISTRY,  22  (36): (7485-7491).  [PMID:39189395] [10.1039/D4OB00874J]

5. Xiaoyu Cui, Liqing Feng, Jiayin Li, Shan Gao, Shunping Yan, Ting Pan.  (2026)  Chloroplast vesiculation fine-tunes seed germination by antagonizing ABA signaling through dual degradation of ABI5 and XIW1.  PLANT JOURNAL,  125  (1): (e70669).  [PMID:41525299] [10.1111/tpj.70669]