GRADE & PURITYCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.Azide Free?Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory.High Performance?High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short.PBS Only?PBS-only formulation — supplied in phosphate-buffered saline with no other additives. Use when you need a clean PBS buffer free of stabilizers/carriers.≥95%(SDS-PAGE)1.0 mg/mL
Azide Free, Carrier Free, ExactAb™, High Performance, PBS Only, Validated
Isotype
Mouse IgG1
Light Chain Type
kappa
SDS-PAGE
150.0 KDa
Purification Method
Protein A purified
Concentration
1.0 mg/mL
Note
This product can replace Ab105284
Storage and Shipping
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Images
GFP/EGFP Mouse mAb (Ab186981) - Western Blot All lanes: GFP/EGFP Mouse Antibody (Ab186981) at 1/10000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 40 kDa, 54 kDa, 29kDa Observed band size: 41 kDa, 51 kDa, 30&27 kDa Exposure time: 21.2 s
GFP/EGFP Mouse mAb (Ab186981) - Western Blot All lanes: GFP/EGFP Mouse Antibody (Ab186981) at 1/10000 dilution Samples: Recombinant GFP Protein (rp156636) at 10 ng per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 28 kDa Observed band size: 29 kDa Exposure time: 79.2 s
GFP/EGFP Mouse Antibody (Ab186981) - IP All lanes: GFP/EGFP Mouse Antibody (Ab186981) at 1/5000 dilution Lane1: HEK-293T transfected with an empty vector, whole cell lysate Lane2: HEK-293T transfected with an empty vector containing an EGFP-myc-tag, whole cell lysate Lane3: Anti-GFP Nanobody Magnetic Beads (G1373495) IP in HEK-293T transfected with an empty vector, whole cell lysate Lane4: Anti-GFP Nanobody Magnetic Beads (G1373495) IP in HEK-293T transfected with an empty vector containing an EGFP-myc-tag, whole cell lysate Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 27 kDa Observed band size: 27, 29 kDa Exposure time: 30.0 s
Application
Application
Dilution info
WB
1/1000 - 1/2000
IP
1/5000
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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