HEPES sodium salt(HEPES-Na) - for cell culture, ≥99.5%(T) , CAS No.75277-39-3

CAS: 75277-39-3 Cat. No.: H100306 Molecular Weight: 260.29 EC Number: 278-169-7

GRADE & PURITY for Cell culture, ≥99.5%(T)

Synonyms

FT-0610868 | HEPES sodium salt | Sodium 2-(4-(2-hydroxyethyl)piperazin-1-yl)ethanesulfonate | N-(2-Hydroxyethyl)piperazine-N'-2-ethanesulfonic acid,sodium salt | AKOS015897769 | Z9FTO91O8A | N-2-Hydroxyethylpiperazine-N'-2-ethanesulphonic acid hemisodium

Storage

Room temperature

Shipped In

Normal

Overview

HEPES, or 4-(2-hydroxyethyl)piperazine-1-ethane-sulfonic acid, is a zwitterionic N-substituted aminosulfonic acid buffer with a remarkable pH buffering capacity between 6.8 and 8.2 (pKa of 7.48 at 25°C). Widely recognized for its versatility, HEPES is extensively employed in cell biology, biochemical, and biological research. Notably, its neutrality towards metal ions makes it a preferred choice for solutions with metal ions, acting as a "non-coordinating buffer." In cell culture, HEPES excels in maintaining stable physiological pH even amidst fluctuating carbon dioxide concentrations, outperforming bicarbonate buffers (NaHCO3). Moreover, HEPES showcases valuable properties in various biological and biochemical processes, such as isoelectric focusing for protein separation and minimal interference in DNA-restriction enzyme reactions, making it indispensable across diverse research applications, including immunoprecipitation, cell lysis, and live cell imaging. Its versatility, exceptional buffering capacity, and minimal interaction with molecules establish HEPES as a key tool in various research domains.

Specifications

Synonyms

FT-0610868 | HEPES sodium salt | Sodium 2-(4-(2-hydroxyethyl)piperazin-1-yl)ethanesulfonate | N-(2-Hydroxyethyl)piperazine-N'-2-ethanesulfonic acid, sodium salt | AKOS015897769 | Z9FTO91O8A | N-2-Hydroxyethylpiperazine-N'-2-ethanesulphonic acid hemisodium

Specifications & Purity

for cell culture, ≥99.5%(T)

Storage

Room temperature

Shipped In

Normal

Grade

for Cell culture

Purity

≥99.5%(T)

Names and Identifiers

• Pubchem Sid: 504760869
• Pubchem Sid Url: https://pubchem.ncbi.nlm.nih.gov/substance/504760869
• Canonical Smiles: C1CN(CCN1CCO)CCS(=O)(=O)[O-].[Na+]
• IUPAC Name: sodium;2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonate
• InChIKey: RDZTWEVXRGYCFV-UHFFFAOYSA-M
• INCHI: 1S/C8H18N2O4S.Na/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14;/h11H,1-8H2,(H,12,13,14);/q;+1/p-1
• Isomeric SMILES: C1CN(CCN1CCO)CCS(=O)(=O)[O-].[Na+]
• WGK Germany: 3
• Molecular Weight: 260.29
• Reaxy-Rn: 14541911
• Reaxys-RN_link_address: https://www.reaxys.com/reaxys/secured/hopinto.do?context=S&query=IDE.XRN=14541911&ln=

Taxonomic Classification

Taxonomy Tree

• Kingdom Organic compounds
  • Superclass Organoheterocyclic compounds
    • Class Diazinanes
      • Subclass Piperazines

• Kingdom: Organic compounds
• Superclass: Organoheterocyclic compounds
• Class: Diazinanes
• Subclass: Piperazines
• Intermediate Tree Nodes: Not available
• Direct Parent: N-alkylpiperazines
• Alternative Parents: Sulfonyls Organosulfonic acids Alkanesulfonic acids Trialkylamines 1,2-aminoalcohols Azacyclic compounds Primary alcohols Organopnictogen compounds Organic zwitterions Organic sodium salts Organic oxides Hydrocarbon derivatives
• Molecular Framework: Aliphatic heteromonocyclic compounds
• Substituents: N-alkylpiperazine - Organic sulfonic acid or derivatives - Organosulfonic acid or derivatives - Organosulfonic acid - Sulfonyl - Alkanesulfonic acid - 1,2-aminoalcohol - Tertiary amine - Tertiary aliphatic amine - Alkanolamine - Azacycle - Organic alkali metal salt - Organic nitrogen compound - Organic zwitterion - Organic salt - Organic sodium salt - Hydrocarbon derivative - Organic oxide - Organopnictogen compound - Primary alcohol - Organosulfur compound - Organooxygen compound - Organonitrogen compound - Amine - Organic oxygen compound - Alcohol - Aliphatic heteromonocyclic compound
• Description: This compound belongs to the class of organic compounds known as n-alkylpiperazines. These are organic compounds containing a piperazine ring where the nitrogen ring atom carries an alkyl group.
• External Descriptors: organic sodium salt - N-(2-hydroxyethyl)piperazine - N-(sulfoalkyl)piperazine

Data sources

1. Djoumbou Feunang Y, Eisner R, Knox C, Chepelev L, Hastings J, Owen G, Fahy E, Steinbeck C, Subramanian S, Bolton E, Greiner R, and Wishart DS. ClassyFire: Automated Chemical Classification With A Comprehensive, Computable Taxonomy. Journal of Cheminformatics, 2016, 8:61.

DOI: 10.1186/s13321-016-0174-y

Chemical and Physical Properties

• Solubility: water: 500 mg/mL, clear, colorless
• Sensitivity: Moisture Sensitive
• Molecular Weight: 260.290 g/mol
• Hydrogen Bond Donor Count: 1
• Hydrogen Bond Acceptor Count: 6
• Rotatable Bond Count: 5
• Exact Mass: 260.081 Da
• Monoisotopic Mass: 260.081 Da
• Topological Polar Surface Area: 92.300 Ų
• Heavy Atom Count: 16
• Formal Charge: 0
• Complexity: 272.000
• Isotope Atom Count: 0
• Defined Atom Stereocenter Count: 0
• Undefined Atom Stereocenter Count: 0
• Defined Bond Stereocenter Count: 0
• Undefined Bond Stereocenter Count: 0
• The total count of all stereochemical bonds: 0
• Covalently-Bonded Unit Count: 2

XLogP3

References

1. Xiaoxue Song, Huanbin Li, Weijun Tong, Changyou Gao.  (2013)  Fabrication of triple-labeled polyelectrolyte microcapsules for localized ratiometric pH sensing.  JOURNAL OF COLLOID AND INTERFACE SCIENCE,      [PMID:24370429] [10.1016/j.jcis.2013.11.008]

2. Ping Wang, Boyuan Liu, Qingqing Wang, Yue Wang, Xiuqian Gao, Jingxin Gou, Haibing He, Yu Zhang, Tian Yin, Xiangqun Jin, Xing Tang.  (2024)  Enhanced localized therapeutic precision: A face-to-face folate-targeted Cu2+-mediated nanotherapy with thermosensitive sustained-release system.  INTERNATIONAL JOURNAL OF PHARMACEUTICS,      [PMID:38729382] [10.1016/j.ijpharm.2024.124213]

3. Yanju Chen, Hui Wu, Siwenjie Qian, Xiaoping Yu, Huan Chen, Jian Wu.  (2021)  Applying CRISPR/Cas system as a signal enhancer for DNAzyme-based lead ion detection.  ANALYTICA CHIMICA ACTA,      [PMID:35057922] [10.1016/j.aca.2021.339356]