Recombinant Human FGF basic/FGF2/bFGF(155aa) Protein, ＞95% SDS-PAGE, CAS No.62031-54-3

CAS: 62031-54-3 Cat. No.: rp145939 Molecular Weight: 17kD

GRADE & PURITY Animal Free, Carrier Free, Bioactive, ActiBioPure™, High Performance, PBS Only, ≥95%(SDS-PAGE)

Expression System

E.coli

Accession #

P09038

Protein Tag

No tag

Expression system

E. coli

Endotoxin Concentration

<0.2 EU/μg

Bioactivity

Measured in a cell proliferation assay using BALB/3T3 mouse embryo fibroblasts cell line. The ED₅₀ for this effect is typically <0.9 ng/mL.

Overview

Recombinant human basic fibroblast growth factor (also known as basic FGF, bFGF, FGF2, FGF-beta, or heparin-binding growth factor), is a biologically active protein suitable for cell culture applications. bFGF regulates diverse processes such as cell proliferation, differentiation, survival, adhesion, motility, apoptosis, limb formation, and wound recovery. bFGF can be used in studies of angiogenesis, fibroblast mitosis, axonal outgrowth in PC-12 cells, receptor binding, and tyrosine phosphorylation. This strain is expressed in recombinant Escherichia coli, and after multi-step separation and purification, it is dissolved in 10mM PBS, 0.15 M NaCl (pH7.2) solution, filtered through a 0.22 μm filter membrane, and then freeze-dried to make a lyophilized powder.

Specifications

Product Name

Recombinant Human FGF basic/FGF2/bFGF(155aa) Protein, ＞95% SDS-PAGE, CAS No.62031-54-3

Synonyms

Basic fibroblast growth factor Basic fibroblast growth factor bFGF BFGF FGF 2 FGF B FGF-2 Fgf2 FGF2 basic FGF2_HUMAN FGFB Fibroblast growth factor 2 Fibroblast growth factor 2 (basic) Fibroblast growth factor, basic HBGF 2 HBGF-2 HBGF2 HBGH 2 HBGH2 Hepari

Grade

ActiBioPure™, Animal Free, Bioactive, Carrier Free, High Performance, PBS Only

Specifications & Purity

Animal Free, Carrier Free, Bioactive, ActiBioPure™, High Performance, PBS Only, ≥95%(SDS-PAGE)

Purity

＞95% SDS-PAGE

Bioactivity

Measured in a cell proliferation assay using BALB/3T3 mouse embryo fibroblasts cell line. The ED₅₀ for this effect is typically <0.9 ng/mL.

Endotoxin Concentration

<0.2 EU/μg

Expression System

E.coli

Species

Human

Amino Acids

134-288 aa

Sequence

MAAGSITTLPALPEDGGSGAFPPGHFKDPKRLYCKNGGFFLRIHPDGRVDGVREKSDPHIKLQLQAEERGVVSIKGVCANRYLAMKEDGRLLASKCVTDECFFFERLESNNYNTYRSRKYTSWYVALKRTGQYKLGSKTGPGQKAILFLPMSAKS

Protein Tag

No tag

Protein Length

Full length protein

Accession #

P09038

Source

Recombinant

Predicted molecular weight

17 kDa

CAS

62031-54-3

Molecule Type

Protein

Storage and Shipping

Shape

Lyophilized

Reconstitution

It is recommended to briefly centrifuge before opening the cap to make the powder sink to the bottom of the tube, then dissolve it in sterilized deionized water to prepare a 100μg/ml stock solution, which can be stored stably for one week at 2°C ~ 8°C. When diluting to a lower concentration (not lower than 10μg/ml), 0.1% tissue culture grade BSA or HSA needs to be added. If extended storage time is required, it is recommended to freeze at -80°C or -20°C, repeated freezing and thawing is not recommended.

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at 2-8℃ short term (4 weeks). Store at -20℃ long term (24 months). Upon reconstitution, it is recommended to aliquot. Avoid freeze/thaw cycle.

Images

SDS-PAGE: Recombinant Human FGF basic/FGF2/bFGF(155aa) Protein (rp145939)

Recombinant Human FGF basic/FGF2/bFGF(155aa) Protein (rp145939)-SDS-PAGE
2μg/lane of Recombinant Human FGF2 was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a single band at 15.6 kDa.

Protein Bioactivity: Recombinant Human FGF basic/FGF2/bFGF(155aa) Protein (rp145939)

Recombinant Human FGF basic/FGF2/bFGF(155aa) Protein (rp145939)-Protein Bioactivity
Measured in a cell proliferation assay using BALB/3T3 mouse embryo fibroblasts cell line. The ED₅₀ for this effect is typically <0.9ng/mL.

References

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