UltraPure dTTP (100mM) - BioReagent, DNase, RNase free, PCR Reagent, endotoxin tested, UltraBio™, Suitable for molecular biology, sterile, for DNA and RNA applications, ≥99%, 100mM , CAS No.18423-43-3

CAS: 18423-43-3 Cat. No.: U759852 Formula: C₁₀H₁₃N₂O₁₄P₃Na₃·2H₂O Molecular Weight: 548.11 EC Number: 838-835-5

GRADE & PURITY BioReagent, DNase, RNase free, PCR Reagent, Endotoxin tested, UltraBio™, Suitable for molecular biology, Sterile, for DNA and RNA applications, ≥99%, 100mM

Synonyms

Deoxythymidine 5'-triphosphate|DTTP

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Overview

Physical appearance:Liquid
Storage buffer:Ultrapure water

Description of products:
dTTP (100mM, UltraPure) is a single-component of ultrapure dTTP, which is suitable for use in PCR, real-time PCR, RT-PCR, cDNA or DNA synthesis, primer extension reaction, DNA sequencing, DNA labeling, and other routine molecular biology reactions that require high-purity dNTP. The pH is titrated to 7.0-7.5 with NaOH solution. dNTP (Deoxy-ribonucleoside triphosphate) includes dATP (deoxyadenosine triphosphate), dCTP (deoxycytidine triphosphate), dGTP (deoxyguanosine triphosphate), dTTP (deoxythymidine triphosphate), and dUTP (deoxyuridine triphosphate). Among them, dATP, dCTP, dGTP and dTTP are indispensable in the PCR reaction as raw materials. DNA polymerase synthesizes a new DNA strand complementary to the DNA template strand by adding dNTPs that are complementary to the template in 5′ to 3′ direction, and thus the quality and concentration of dNTP are closely related to the amplification efficiency.
Precautions:
This product can be thawed at room temperature. Keep it on ice for use and store at -20℃ immediately after use. This product is for research use only, not for drug, clinical diagnosis, food, or other uses. For your safety and health, please wear a lab coat and disposable gloves during the operation.

Specifications

Synonyms

Deoxythymidine 5'-triphosphate | DTTP

Specifications & Purity

BioReagent, DNase, RNase free, PCR Reagent, endotoxin tested, UltraBio™, Suitable for molecular biology, sterile, for DNA and RNA applications, ≥99%, 100mM

Stability And Storage

Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

This product requires cold chain shipping. Ground and other economy services are not available.

Grade

BioReagent, DNase, RNase free, Endotoxin tested, for DNA and RNA applications, PCR Reagent, Sterile, Suitable for molecular biology, UltraBio™

Purity

≥99%

Product Line

Biological Products

Names and Identifiers

PH

7.0-7.5

Canonical Smiles

CC1=CN(C(=O)NC1=O)C2CC(C(O2)COP(=O)([O-])OP(=O)([O-])OP(=O)(O)[O-])O.[Na+].[Na+].[Na+]

IUPAC Name

trisodium;[[[(2R,3S,5R)-3-hydroxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-oxidophosphoryl]oxy-oxidophosphoryl] hydrogen phosphate

InChIKey

KNVJTOALWGZWAI-SPSULGLQSA-K

INCHI

1S/C10H17N2O14P3.3Na/c1-5-3-12(10(15)11-9(5)14)8-2-6(13)7(24-8)4-23-28(19,20)26-29(21,22)25-27(16,17)18;;;/h3,6-8,13H,2,4H2,1H3,(H,19,20)(H,21,22)(H,11,14,15)(H2,16,17,18);;;/q;3*+1/p-3/t6-,7+,8+;;;/m0.../s1

Isomeric SMILES

CC1=CN(C(=O)NC1=O)[C@H]2C[C@@H]([C@H](O2)COP(=O)([O-])OP(=O)([O-])OP(=O)(O)[O-])O.[Na+].[Na+].[Na+]

Molecular Weight

548.11

Taxonomic Classification

Taxonomy Tree

• Kingdom Organic compounds
  • Superclass Nucleosides, nucleotides, and analogues
    • Class Pyrimidine nucleotides
      • Subclass Pyrimidine deoxyribonucleotides
        • Level5 Pyrimidine deoxyribonucleoside triphosphates
          • Level6 Pyrimidine 2'-deoxyribonucleoside triphosphates

• Kingdom: Organic compounds
• Superclass: Nucleosides, nucleotides, and analogues
• Class: Pyrimidine nucleotides
• Subclass: Pyrimidine deoxyribonucleotides
• Intermediate Tree Nodes: Pyrimidine deoxyribonucleoside triphosphates
• Direct Parent: Pyrimidine 2'-deoxyribonucleoside triphosphates
• Alternative Parents: Pyrimidones Alkyl phosphates Hydropyrimidines Vinylogous amides Heteroaromatic compounds Oxolanes Lactams Ureas Secondary alcohols Oxacyclic compounds Azacyclic compounds Organopnictogen compounds Organonitrogen compounds Organic sodium salts Organic oxides Hydrocarbon derivatives Organic cations
• Molecular Framework: Aromatic heteromonocyclic compounds
• Substituents: Pyrimidine 2'-deoxyribonucleoside triphosphate - Pyrimidone - Hydropyrimidine - Organic phosphoric acid derivative - Phosphoric acid ester - Pyrimidine - Alkyl phosphate - Oxolane - Heteroaromatic compound - Vinylogous amide - Lactam - Urea - Secondary alcohol - Organoheterocyclic compound - Azacycle - Organic alkali metal salt - Oxacycle - Organic nitrogen compound - Organic salt - Hydrocarbon derivative - Organic oxide - Organopnictogen compound - Alcohol - Organooxygen compound - Organonitrogen compound - Organic oxygen compound - Organic sodium salt - Organic cation - Aromatic heteromonocyclic compound
• Description: This compound belongs to the class of organic compounds known as pyrimidine 2'-deoxyribonucleoside triphosphates. These are pyrimidine nucleotides with a triphosphate group linked to the ribose moiety lacking a hydroxyl group at position 2.
• External Descriptors: Not available

Data sources

1. Djoumbou Feunang Y, Eisner R, Knox C, Chepelev L, Hastings J, Owen G, Fahy E, Steinbeck C, Subramanian S, Bolton E, Greiner R, and Wishart DS. ClassyFire: Automated Chemical Classification With A Comprehensive, Computable Taxonomy. Journal of Cheminformatics, 2016, 8:61.

DOI: 10.1186/s13321-016-0174-y

Chemical and Physical Properties

• Melt Point(°C): 218 - 220°C

Frequently Asked Questions

What is the purity of this product?

This product is supplied at ≥99% purity (chemical assay). Lot-specific values are stated on the Certificate of Analysis.

What does BioReagent mean?

BioReagent indicates that the product has been processed and tested for biological and biochemical applications. Specifications may include endotoxin limits, microbial controls, sterility, low DNase/RNase/protease activity, and verified biological activity where relevant.

What does DNase, RNase free mean?

DNase, RNase free indicates that the product has been processed and tested for sensitive nucleic acid and protein workflows. Typical specifications include low or undetectable nuclease/protease activity, low endotoxin, controlled microbial limits and high lot-to-lot reproducibility.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What are the CAS number, molecular formula and molecular weight?

The CAS Number is 18423-43-3, the molecular formula is C10H13N2O14P3Na3·2H2O, and the molecular weight is 548.11 g/mol. InChIKey KNVJTOALWGZWAI-SPSULGLQSA-K.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.