Antigen Retrieval Solution for Floating Sections (10×) - BioReagent, sterile-filtered, Suitable for Immunohistochemistry, 10X

Cat. No.: A752121

GRADE & PURITY Sterile-filtered, BioReagent, Suitable for Immunohistochemistry(IHC), 10X

Synonyms

Antigen Retrieval Solution (10X)

Storage

Store at 2-8°C

Shipped In

Wet ice

Overview

Antigen Retrieval Solution for Floating Sections (10×) contains sodium citrate and other antigen repair reagents, pH value is about 8.5, can effectively reduce the risk of bleach breakage and remove the cross-linking between proteins caused by aldehyde fixing reagents, fully expose the antigen epitope in paraffin sections and other samples, thus greatly improving the immunostaining effect. It is suitable for the antigen repair of bleaching pieces, and can also be used for the antigen repair of paraffin sections, frozen sections and other samples.
Operation process:
1. Before the reagent is ready to use, the antigen repair solution (10×) must be diluted 10 times with distilled water or ultra-pure water to prepare the antigen repair solution (1×).
2. Antigen Repair
2.1 Paraffin section:
2.1.1 Dewaxing: section dewaxing in xylene for 2×5min. Anhydrous ethanol 2×5min. 90% ethanol 2×5min, 70% ethanol 5min. Distilled water 2×5min.
2.1.2 Antigen repair: The section is immersed in antigen repair solution (1×) and heated at 80°C for about 30min(the optimal heating time should be determined according to different samples and target proteins).
Note: Antigen repair solution (1×) should be preheated to 80°C before use. Can be heated in a water bath. Then cool to room temperature in about 20 to 30 minutes. Wash with immunostaining detergent 1~2 times, 3~5min each time. Subsequent immunostaining steps such as blocking can be performed.
2.2 Frozen section: The section was washed with immunostaining detergent for 5min. The process of antigen repair such as the process of antigen repair in paraffin sections.
2.3 For antigen repair of other samples, please refer to the steps of paraffin section or frozen section.
Tips: For your own safety, please take good protection before using the reagent, such as wearing a lab coat, wearing gloves, etc.

Specifications

Synonyms

Antigen Retrieval Solution (10X)

Specifications & Purity

BioReagent, sterile-filtered, Suitable for Immunohistochemistry, 10X

Stability And Storage

Store at 2-8℃ for 12 months. Store at -20℃ for 24 months. Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Storage

Store at 2-8°C

Shipped In

Wet ice

This product requires cold chain shipping. Ground and other economy services are not available.

Grade

BioReagent, Sterile-filtered, Suitable for Immunohistochemistry(IHC)

Names and Identifiers

• PH: 8.5

Frequently Asked Questions

What does sterile-filtered mean?

sterile-filtered indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at 2–8 °C. Refrigerated storage is required to maintain the specified shelf life.

What does BioReagent mean?

BioReagent indicates that the product has been processed and tested for biological and biochemical applications. Specifications may include endotoxin limits, microbial controls, sterility, low DNase/RNase/protease activity, and verified biological activity where relevant.

How is this product shipped?

This product ships chilled on wet ice. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.