BIS-TRIS propane - Ultra pure, ≥98%(T), non-zwitterionic buffer useful in pH range 6.3-9.5 , CAS No.64431-96-5

CAS: 64431-96-5 Cat. No.: B755526 Molecular Weight: 282.33 Beilstein Registry Number: 1786109 EC Number: 264-899-3

GRADE & PURITY Ultra pure, ≥98%(T), non-zwitterionic buffer useful in pH range 6.3-9.5

Storage

Room temperature,Argon charged

Shipped In

Normal

Overview

Bis-Tris propane buffer, also known as BTP, is a zwitterionic biochemical buffer used for pH adjustment in various laboratory applications, particularly molecular biology, biochemistry, and electrophoresis. Its wide buffering range, from pH 6.3 to 9.5, is attributed to its close pKa values ​​of 6.8 (pKa1) and 9.0 (pKa2). This broad buffering range allows it to maintain stable pH conditions in a variety of experiments.

Furthermore, Bis-Tris propane is a water-soluble buffer that plays a crucial role in improving the stability and activity of restriction enzymes. Specifically, its buffering capacity is advantageous for experiments requiring pH levels as low as 6-7. Tris buffers are insufficient at pH below 7.5 and exhibit significant pKa fluctuations with temperature changes; Bis-Tris propane is a better alternative in this regard. Additionally, it can be used in conjunction with hydrochloric acid buffer to stabilize farnesyl diphosphate, particularly during its isolation from Saccharomyces cerevisiae. In particular, it can act as a ligand and form binuclear hydroxyl complexes with lanthanide (III) rare earth elements.

Application:

• Comparing solution-gate and bottom-gate nanowire field-effect transistors on pH sensing with different salt concentrations and surface modifications.: BIS-TRIS propane is used as a buffering agent in the development of nanowire field-effect transistors for pH sensing. The research demonstrates its effectiveness in enhancing the sensitivity and stability of these biosensors under varying conditions (Hu et al., 2024).


• Laser-induced tuning of graphene field-effect transistors for pH sensing.: This study employs BIS-TRIS propane as a buffering solution to optimize the performance of graphene field-effect transistors in pH sensing applications. The findings highlight the role of BIS-TRIS propane in achieving precise tuning of sensor responses (Lampinen et al., 2023).


• Elucidating the exchange interactions in a {Gd(III)Cu(II)(4)} propellor.: BIS-TRIS propane is utilized as a buffer in the investigation of exchange interactions in complex metal assemblies. The study provides insights into the magnetic properties and potential applications in materials science (Heras Ojea et al., 2023).


• Protein association on multimodal chromatography media.: This research explores the use of BIS-TRIS propane in multimodal chromatography to study protein interactions. The buffer′s compatibility with various chromatographic techniques enhances the understanding of protein behavior and purification processes (Muca & Antos, 2023).

Specifications

Specifications & Purity

Ultra pure, ≥98%(T), non-zwitterionic buffer useful in pH range 6.3-9.5

Storage

Room temperature, Argon charged

Shipped In

Normal

Grade

Ultra pure

Purity

≥98%(T)

Names and Identifiers

• Canonical Smiles: C(CNC(CO)(CO)CO)CNC(CO)(CO)CO
• IUPAC Name: 2-[3-[[1,3-dihydroxy-2-(hydroxymethyl)propan-2-yl]amino]propylamino]-2-(hydroxymethyl)propane-1,3-diol
• InChIKey: HHKZCCWKTZRCCL-UHFFFAOYSA-N
• INCHI: 1S/C11H26N2O6/c14-4-10(5-15,6-16)12-2-1-3-13-11(7-17,8-18)9-19/h12-19H,1-9H2
• Isomeric SMILES: C(CNC(CO)(CO)CO)CNC(CO)(CO)CO
• WGK Germany: 3
• Molecular Weight: 282.33
• Beilstein: 1786109
• Reaxy-Rn: 1786109
• Reaxys-RN_link_address: https://www.reaxys.com/reaxys/secured/hopinto.do?context=S&query=IDE.XRN=1786109&ln=

Taxonomic Classification

Taxonomy Tree

• Kingdom Organic compounds
  • Superclass Organic nitrogen compounds
    • Class Organonitrogen compounds
      • Subclass Amines
        • Level5 Alkanolamines
          • Level6 1,2-aminoalcohols

• Kingdom: Organic compounds
• Superclass: Organic nitrogen compounds
• Class: Organonitrogen compounds
• Subclass: Amines
• Intermediate Tree Nodes: Alkanolamines
• Direct Parent: 1,2-aminoalcohols
• Alternative Parents: Dialkylamines Primary alcohols Organopnictogen compounds Hydrocarbon derivatives
• Molecular Framework: Aliphatic acyclic compounds
• Substituents: 1,2-aminoalcohol - Secondary amine - Secondary aliphatic amine - Organic oxygen compound - Organopnictogen compound - Hydrocarbon derivative - Primary alcohol - Organooxygen compound - Alcohol - Aliphatic acyclic compound
• Description: This compound belongs to the class of organic compounds known as 1,2-aminoalcohols. These are organic compounds containing an alkyl chain with an amine group bound to the C1 atom and an alcohol group bound to the C2 atom.
• External Descriptors: hexol

Data sources

1. Djoumbou Feunang Y, Eisner R, Knox C, Chepelev L, Hastings J, Owen G, Fahy E, Steinbeck C, Subramanian S, Bolton E, Greiner R, and Wishart DS. ClassyFire: Automated Chemical Classification With A Comprehensive, Computable Taxonomy. Journal of Cheminformatics, 2016, 8:61.

DOI: 10.1186/s13321-016-0174-y

Chemical and Physical Properties

• Solubility: water: 1 M
• Sensitivity: Moisture sensitive
• Melt Point(°C): 164-165°C
• Molecular Weight: 282.330 g/mol
• XLogP3: -4.600
• Hydrogen Bond Donor Count: 8
• Hydrogen Bond Acceptor Count: 8
• Rotatable Bond Count: 12
• Exact Mass: 282.179 Da
• Monoisotopic Mass: 282.179 Da
• Topological Polar Surface Area: 145.000 Ų
• Heavy Atom Count: 19
• Formal Charge: 0
• Complexity: 183.000
• Isotope Atom Count: 0
• Defined Atom Stereocenter Count: 0
• Undefined Atom Stereocenter Count: 0
• Defined Bond Stereocenter Count: 0
• Undefined Bond Stereocenter Count: 0
• The total count of all stereochemical bonds: 0
• Covalently-Bonded Unit Count: 1

References

1. Jie Gu, Shuyun Zheng, Xiaolong Lu, Hao Zhang, Kai Ren, Ziqiang Liu, Chao Liu, Chunrui Wu.  (2024)  A new strategy for solving hydrophobic membrane wetting: membrane structure design for membrane pore drying by spontaneous dehydration.  JOURNAL OF MEMBRANE SCIENCE,      [PMID:] [10.1016/j.memsci.2024.123664]

2. Jiandong Shen, Bijiang Zhong, Wenshui Xia, Yanshun Xu.  (2024)  Action of structural proteins in textural deterioration of grass carp (Ctenopharyngodon idellus) fillets during refrigerated storage.  INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY,  59  (4): (2659-2666).  [PMID:] [10.1111/ijfs.17010]

3. Seokjoon Kim, Seungjin Lee, Seokhwan Kim, Jiye Shin, Byung Seok Cha, Eun Sung Lee, Ki Soo Park.  (2024)  Colorimetric detection of arsenite using Tris-mediated gold nanoparticle aggregation and chitosan lateral flow strip-based signal enhancement.  SENSORS AND ACTUATORS B-CHEMICAL,      [PMID:] [10.1016/j.snb.2024.135469]

4. Bao-Di Ma, Jia-Yi Li, Jian-He Xu, Tao Yu, Xu-Dong Kong.  (2025)  ADP-ribose is a competitive inhibitor of methanol dehydrogenases from Bacillus methanolicus.  JOURNAL OF BIOLOGICAL CHEMISTRY,      [PMID:40818608] [10.1016/j.jbc.2025.110599]

5. Yu Han, Yuelin Luo, Bao-Di Ma, Jie Li, Jian-He Xu, Xu-Dong Kong.  (2024)  Structural Insights of a cis-Epoxysuccinate Hydrolase Facilitate the Development of Robust Biocatalysts for the Production of l-(+)-Tartrate.  BIOCHEMISTRY,      [PMID:38803051] [10.1021/acs.biochem.4c00141]