BSA Blocking Buffer

Cat. No.: B670017

Synonyms

Blocking Western

Storage

Store at 2-8°C

Shipped In

Wet ice

Overview

Product Introduction:

In Western Blot experiments, in order to prevent non-specific adsorption between the imprinting membrane and protein components in the detection system (such as antibodies IgG, etc.), which may lead to unclear experimental results, it is usually necessary to block the non-specific binding sites on the imprinting membrane before incubating with primary antibodies. This product is based on BSA and combined with other active ingredients to quickly and efficiently seal excess binding sites on the membrane, reducing the occurrence of non-specific binding and lowering the background. It is suitable for sealing various transfer films and is easy and fast to operate. In addition, this product has specially added protein stabilizers to ensure stable and reliable sealing effects in each experiment.

Precautions：

1. During each use, it is necessary to ensure that the sealing solution completely submerges the imprinting film to avoid affecting the sealing effect.
2. This product contains preservatives, please wear gloves for protection during operation.
3. This product is only used for scientific research and cannot be used for human experiments or treatments.

Instructions for Use：

1. This product does not require dilution and can be used immediately after opening the lid.
2. After the transfer of the target protein is completed, place the imprinting membrane into a container and add an appropriate amount of BSA Blocking Buffer (preferably to completely cover the imprinting membrane).
3. Incubate at room temperature for 30 minutes to 2 hours, and if necessary, incubate overnight at 2-8 ℃.
4. After the closure is completed, discard the BSA Blocking Buffer and perform subsequent Western Blot operations.

Specifications

Synonyms

Blocking Western

Stability And Storage

Store at 2-8°C long term (12 months). Upon delivery aliquot.

Storage

Store at 2-8°C

Shipped In

Wet ice

This product requires cold chain shipping. Ground and other economy services are not available.

References

1. Ling Jun, Zhang Jie, Wang Baozhen, FengSong Jing, Li Tao, Chen Jing.  (2025)  Artificial intelligence-driven discovery of YH395A: A novel TGFβR1 inhibitor with potent anti-tumor activity against triple-negative breast cancer.  Cell Communication and Signaling,  23  (1): (1-19).  [PMID:40629347] [10.1186/s12964-025-02337-2]