CD122/IL-2Rβ Rat mAb (Biotin) - Primary antibody, specific to CD122/IL-2Rβ, Rat IgG2b

Cat. No.: Ab185171

GRADE & PURITY Validated, ExactAb™, 0.5 mg/mL

Application

Flow Cytometry

Host species

Rat

Reactive species

Mouse

Isotype

Rat IgG2b

Clone Number

TM-β1

Conjugation

Biotin

Overview

Rat anti Mouse CD122/IL-2Rβ Antibody, Monoclonal (TM-β1), could be used for Flow and so on.

Specifications

Product Name

CD122/IL-2Rβ Rat mAb (Biotin) - Primary antibody, specific to CD122/IL-2Rβ, Rat IgG2b

Synonyms

CD122 antigen | CD122 | high affinity IL-2 receptor beta subunit | High affinity IL-2 receptor subunit beta | IL-15 R beta | IL-2 R beta | IL-2 receptor subunit beta | IL2R beta | IL-2R subunit beta | IL2RB | IL-2Rb | interleukin 15 receptor, beta | inter

Specifications & Purity

ExactAb™, Validated, 0.5 mg/mL

Biochemical and Physiological Mechanisms

Receptor for interleukin-2. This beta subunit is involved in receptor mediated endocytosis and transduces the mitogenic signals of IL2.

Host species

Rat

Specificity

CD122/IL-2Rβ

Immunogen

Recombinant fragment corresponding to Human IL2 Receptor beta/p75 aa 1-250. Expressed in E.coli.

Positive Control

Flow: C57BL/6 mouse splenocytes.

Conjugation

Biotin

Grade

ExactAb™, Validated

Isotype

Rat IgG2b

Light Chain Type

kappa

Purification Method

Protein G purified

Concentration

0.5 mg/mL

Storage and Shipping

Storage

Store at 2-8°C,Protected from light,Do not freeze

Shipped In

Wet ice,Do not freeze

Stability And Storage

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Images

Flow Cytometry: CD122/IL-2Rβ Rat mAb (Biotin) (Ab185171)

CD122/IL-2Rβ Rat mAb (Biotin) (Ab185171) - Flow Cytometry
Flow cytometry analysis of CD122/IL-2Rβ expression by C57BL/6 mouse splenocytes (right panel) compared to an isotype control (left panel). Cells were stained with CD122/IL-2Rβ Rat mAb (Biotin) (Ab185171) at 0.3μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Left panel - Rat IgG Isotype Control Antibody (Biotin) (Ab180310).

Flow Cytometry: CD122/IL-2Rβ Rat mAb (Biotin) (Ab185171)

CD122/IL-2Rβ Rat mAb (Biotin) (Ab185171) - Flow Cytometry
Flow cytometry analysis of CD122/IL-2Rβ expression by C57BL/6 mouse splenocytes (red) compared to an isotype control (blue). Cells were stained with CD122/IL-2Rβ Rat mAb (Biotin) (Ab185171) at 0.3μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Blue - Rat IgG Isotype Control Antibody (Biotin) (Ab180310). Black - Unlabelled control, cells without incubation with primary antibody.

Application

• Application: Dilution info
• Flow Cytometry:

  ≤0.5μg/test

  For flow cytometric staining, the suggested use of this reagent is ≤0.5 μg per million cells in 100 µL staining volume.;

Associated Targets(Human)

IL2RB Tclin Interleukin-2 receptor subunit beta (0 Activities)

Discover Target: IL2RB

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mechanisms of Action