CD44 Rat mAb (Biotin) - Primary antibody, specific to CD44

Cat. No.: Ab190173

GRADE & PURITY Validated, ExactAb™, 0.5 mg/mL

Application

Flow Cytometry

Host species

Rat

Reactive species

Mouse

Isotype

Rat IgG2b

Clone Number

IM7

Conjugation

Biotin

Overview

Rat anti Mouse CD44 Antibody, Monoclonal (IM7), could be used for Flow Cytometry.

Specifications

Product Name

CD44 Rat mAb (Biotin) - Primary antibody, specific to CD44

Synonyms

Ly-24 | Pgp-1 | HERMES | CD44 antigen | CD44 molecule (Indian blood group) | CD44 | CD44R | CDw44 | cell surface glycoprotein CD44 | chondroitin sulfate proteoglycan 8 | CSPG8 | ECMR-III | epican | Extracellular matrix receptor III | GP90 lymphocyte homin

Specifications & Purity

ExactAb™, Validated, 0.5 mg/mL

Biochemical and Physiological Mechanisms

Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA

Host species

Rat

Specificity

CD44

Immunogen

Dexamethasone-induced myeloid leukemia M1 cells

Positive Control

Flow Cytometry: C57BL/6 mouse splenocytes

Conjugation

Biotin

Grade

ExactAb™, Validated

Light Chain Type

kappa

Purification Method

Protein G purified

Concentration

0.5 mg/mL

Storage and Shipping

Storage

Store at 2-8°C,Protected from light,Do not freeze

Shipped In

Wet ice,Do not freeze

Stability And Storage

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Images

Flow Cytometry: CD44 Rat mAb (Biotin) (Ab190173)

CD44 Rat mAb (Biotin) (Ab190173) - Flow Cytometry
Flow cytometry analysis of CD44 expression by C57BL/6 mouse splenocytes (right panel) compared to an isotype control (left panel). Cells were stained with CD44 Rat mAb (Biotin) (Ab190173) at 0.04μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Left panel - Rat IgG (Biotin) (Ab180310).

Flow Cytometry: CD44 Rat mAb (Biotin) (Ab190173)

CD44 Rat mAb (Biotin) (Ab190173) - Flow Cytometry
Flow cytometry analysis of CD44 expression by C57BL/6 mouse splenocytes (red) compared to an isotype control (blue). Cells were stained with CD44 Rat mAb (Biotin) (Ab190173) at 0.04μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Blue - Rat IgG (Biotin) (Ab180310). Black - Unlabelled control, cells without incubation with primary antibody.

Application

• Application: Dilution info
• Flow Cytometry:

  ≤0.3 μg/test. For flow cytometric staining, the suggested use of this reagent is ≤0.3 μg per million cells in 100 µL staining volume.;