CD47 Rat mAb (AF647) - Primary antibody, specific to CD47, Rat IgG2a

Cat. No.: Ab190282
AVAILABLE TO ORDER
GRADE & PURITY Validated ? Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated. ExactAb™ ? ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications. Ex:650nm ? Fluorescence excitation maximum at 650 nm — the wavelength of light that best excites this fluorophore. Match it to your instrument's laser or filter set for the strongest signal. Em:668nm ? Fluorescence emission maximum at 668 nm — the wavelength this fluorophore emits after excitation. Use it to pick the right detection filter and avoid channel cross-talk. 0.5 mg/mL
Application
Flow Cytometry
Host species
Rat
Reactive species
Mouse
Isotype
Rat IgG2a
Clone Number
MIAP301
Conjugation
AF647
 ·  off list, applied to all prices below.
Size
Status
Price
Qty
10μg
Ab190282-10μg
4
$167.90
25μg
Ab190282-25μg
2
$335.90
100μg
Ab190282-100μg
1
$717.90
Enter a quantity for the sizes you want to add.
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Why this grade

ExactAb™, Validated, Ex:650nm, Em:668nm, 0.5 mg/mL Em:668nm,Ex:650nm,ExactAb™,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

🌡

Storage & shipping

Store at 2-8°C,Protected from light,Do not freeze Ships Wet ice,Do not freeze Check lot-specific COA for exact specifications.

📋

Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

📚

Literature proof

Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

Overview

Rat anti Mouse CD47 Antibody, Monoclonal (MIAP301), could be used for Flow, in vivo CD47 blockade, in vitro CD47 blockade, IF, ICC and so on.


Specifications

Product Name
CD47 Rat mAb (AF647) - Primary antibody, specific to CD47, Rat IgG2a
Synonyms
Itgp | 9130415E20Rik | B430305P08Rik | antigen identified by monoclonal 1D8 | Antigenic surface determinant protein OA3 | CD47 antigen (Rh-related antigen, integrin-associated signal transducer) | CD47 antigen | CD47 glycoprotein | CD47 molecule | CD47 |
Specifications & Purity
ExactAb™, Validated, Ex:650nm, Em:668nm, 0.5 mg/mL
Biochemical and Physiological Mechanisms
Has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. Plays an important role in memory formation and synaptic plasticity in the hippocampus (By similarity). Receptor for SIRPA, bind
Host species
Rat
Specificity
CD47
Immunogen
Intact CD47 purified from placenta
Positive Control
Flow: C57BL/6 mouse splenocytes.
Conjugation
AF647
Excitation (nm)
Red Laser(633nm)
Emission(nm)
668nm
Ex/Em(nm)
Ex:650nm;Em:668nm
Grade
Em:668nm, Ex:650nm, ExactAb™, Validated
Isotype
Rat IgG2a
Light Chain Type
kappa
Purification Method
Protein G purified
Concentration
0.5 mg/mL
Storage and Shipping
Storage
Store at 2-8°C,Protected from light,Do not freeze
Shipped In
Wet ice,Do not freeze
Stability And Storage
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Images

CD47 Rat mAb (AF647) (Ab190282) - Flow Cytometry
Flow cytometry analysis of CD47 expression by C57BL/6 mouse splenocytes (red) compared to an isotype control (blue). Cells were stained with CD47 Rat mAb (AF647) (Ab190282) at 0.3μg/test for 1 hour at 4°C. Blue - Rat IgG (AF647) (Ab190071). Black - Unlabelled control, cells without incubation with primary antibody.

Application
ApplicationDilution info
Flow Cytometry

≤1.0 μg/test, the suggested use of this reagent is ≤1.0 μg per million cells in 100 µL staining volume

Documentation

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

Associated Targets(Human)
CD47 Tchem Leukocyte surface antigen CD47 (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
Mechanisms of Action
Certificates(CoA,COO,BSE/TSE and Analysis Chart)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

3 results found

Lot NumberCertificate TypeDateItem
ZJ24F0911148Certificate of AnalysisApr 07, 2026 Ab190282
ZJ24F0911149Certificate of AnalysisApr 07, 2026 Ab190282
ZJ24F0911150Certificate of AnalysisApr 07, 2026 Ab190282
Documents & Articles
Solution Calculators
Reviews

Customer Reviews

Need help choosing the grade?

Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.

View Validated grade guide → View ExactAb™ grade guide → View Ex:650nm grade guide → View Em:668nm grade guide →

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