CD73 Rat mAb - Primary antibody, specific to CD73, Rat IgG2a

Cat. No.: Ab095255
AVAILABLE TO ORDER
GRADE & PURITY Carrier Free ? Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays. Validated ? Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated. Azide Free ? Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory. PBS Only ? PBS-only formulation — supplied in phosphate-buffered saline with no other additives. Use when you need a clean PBS buffer free of stabilizers/carriers. ≥95%(SDS-PAGE) See COA
Application
Animal Model, Blocking, Flow Cytometry, Functional Assay
Host species
Rat
Reactive species
Mouse
Isotype
Rat IgG2a
Clone Number
TY/23
Conjugation
Unconjugated
 ·  off list, applied to all prices below.
Size
Status
Price
Qty
10μg
Ab095255-10μg
10
$53.90
50μg
Ab095255-50μg
4
$159.90
100μg
Ab095255-100μg
2
$219.90
1mg
Ab095255-1mg
1-2 wks(?)
Item is derived from our semi-finished stock and is processed in 1-2 weeks.
$1,799.90
Enter a quantity for the sizes you want to add.
🧪

Why this grade

Carrier Free,Azide Free,Validated,PBS Only,≥95%(SDS-PAGE),See COA Azide Free,Carrier Free,PBS Only,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

🌡

Storage & shipping

Store at 2-8°C,Do not freeze Ships Wet ice,Do not freeze Check lot-specific COA for exact specifications.

📋

Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

📚

Literature proof

Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

Overview

Rat anti Mouse CD73 Antibody, Monoclonal (TY/23), could be used for Flow, in vivo CD73 blockade, in vitro CD73 blockade and so on.
Application
Flow: Use at an assay dependent concentration.
in vivo CD73 blockade: Use at an assay dependent concentration.
in vitro CD73 blockade: Use at an assay dependent concentration.
Protein Function
Hydrolyzes extracellular nucleotides into membrane permeable nucleosides.

Specifications

Product Name
CD73 Rat mAb - Primary antibody, specific to CD73, Rat IgG2a
Synonyms
Nt5 | 2210401F01Rik | 5' nucleotidase (CD73) | 5'-NT | 5-NT | 5'-Nucleotidase | 5'-nucleotidase, ecto (CD73) | CD73 antigen | CD73 | E5NT | EC 3.1.3.5 | ecto-5'-nucleotidase | eN | eNT | NT | NT55'-nucleotidase | NT5E | NTE | Purine 5-Prime-Nucleotidase |
Specifications & Purity
Carrier Free, Azide Free, Validated, PBS Only, ≥95%(SDS-PAGE), See COA
Host species
Rat
Specificity
CD73
Immunogen
BALB/c mouse spleen cells and CHO cells transfected with the mouse CD73 gene
Conjugation
Unconjugated
Grade
Azide Free, Carrier Free, PBS Only, Validated
Isotype
Rat IgG2a
Light Chain Type
kappa
SDS-PAGE
150 kDa
Purification Method
Protein G purified
Concentration
See COA
Storage and Shipping
Shape
Liquid
Storage
Store at 2-8°C,Do not freeze
Shipped In
Wet ice,Do not freeze
Stability And Storage
Shipped at 4°C. Store at 4°C long term (12 months). Do not freeze. Upon receipt, it is recommended to aliquot.
Images

CD73 Rat mAb (Ab095255) - Flow Cytometry
Flow Cytometry analysis of mouse splenocytes cells labelling CD73 with CD73 Rat mAb (Ab095255) conjugated with biotin (right panel) at 0.5 μg/mL for 1 hour at 4°C, compared with Rat IgG Isotype Control Antibody (Biotin) (Ab180310) (left panel) and detected with SA-PE (rp156150).

CD73 Rat mAb (Ab095255) - Flow Cytometry
Flow Cytometry analysis of mouse splenocytes cells labelling CD73 (red) with CD73 Rat mAb (Ab095255) conjugated with biotin at 0.5 μg/mL for 1 hour at 4°C, and detected with SA-PE (rp156150). Blue - Rat IgG Isotype Control Antibody (Biotin) (Ab180310). Black - Unlabelled control, cells without incubation with primary antibody.

Application
ApplicationDilution info
Flow Cytometry

Use at an assay dependent concentration.

in vivo CD73 blockade

Use at an assay dependent concentration.

in vitro CD73 blockade

Use at an assay dependent concentration.

Documentation

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

Associated Targets(Human)
NT5E Tchem 5'-nucleotidase (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
Mechanisms of Action
Certificates(CoA,COO,BSE/TSE and Analysis Chart)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

7 results found

Lot NumberCertificate TypeDateItem
ZJ25F1230605Certificate of AnalysisDec 26, 2025 Ab095255
ZJ25F1230606Certificate of AnalysisDec 26, 2025 Ab095255
ZJ25F1230604Certificate of AnalysisDec 26, 2025 Ab095255
ZJ25F1230603Certificate of AnalysisDec 26, 2025 Ab095255
ZJ24F0607430Certificate of AnalysisJun 26, 2024 Ab095255
ZJ24F0607429Certificate of AnalysisJun 26, 2024 Ab095255
ZJ24F0607428Certificate of AnalysisJun 26, 2024 Ab095255
Documents & Articles
Solution Calculators
Reviews

Customer Reviews

Need help choosing the grade?

Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.

View Carrier Free grade guide → View Validated grade guide → View Azide Free grade guide → View PBS Only grade guide →

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