dNTP/dUTP Mixture (2.5mM each/5mM) - BioReagent, DNase, RNase free, PCR Reagent, Suitable for molecular biology, sterile, for DNA and RNA applications, ≥99%, dATP、dCTP、dGTP、dTTP: 2.5mM dUTP: 5mM

Cat. No.: D745378

GRADE & PURITY Suitable for molecular biology, BioReagent, DNase, RNase free, PCR Reagent, Sterile, for DNA and RNA applications, ≥99%, dATP、dCTP、dGTP、dTTP: 2.5mM dUTP: 5mM

Synonyms

dNTP/dUTP Mixture | dNTP/dUTP Mix

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Overview

This dNTP/dUTP Mixture (2.5mM each/5mM) is a mixed aqueous solution of dATP, dCTP, dGTP, dTTP and dUTP, where the concentration of dATP, dCTP and dGTP is 2.5mM and the concentration of dUTP is 5mM. This product is commonly used in PCR with Taq DNA polymerase, primer extension and cDNA synthesis. It is more commonly used in conjunction with the UDG enzyme that can recognize and excise deoxyuracil bases to prevent carryover contamination by PCR products from surroundings. This product can be used in PCR with polymerase family A members such as Taq, and also for isothermal amplification with Bst DNA Polymerase. Some enzymes that can use dATP, dTTP, dGTP, dCTP, may not be able to use dUTP. Please refer to manufacture’s instructions of a particular enzyme or make a test.Usually, this product and uracil DNA glycosylase (UDG enzyme) are used in conjunction to establish the dUTP/UDG anti-pollution system in which the UDG enzyme can enzymatically remove possible contamination from previous PCR products containing deoxyuracil (dU), thus effectively minimize cross-over contamination.This dNTP/dUTP Mixture is prepared with ultrapure water and the pH is titrated to 7.0-7.5 with high-purity NaOH solution.This product is free of DNase and RNase, can be directly used for different molecular biology applications.
Precautions：
The UDG enzyme does not work for cases that have been contaminated by dU-free PCR products. In this case, the primers can be redesigned to amplify other regions of target DNA. The dUTP/UDG anti-pollution system should be established to minimize cross-over contamination.This product can be thawed at room temperature. Keep it on ice for use and store at -20℃ immediately after use.This product is for R&D only. Not for drug, household, or other uses.For your safety and health, please wear a lab coat and disposable gloves during the operation.
Instructions for Use：
None

Specifications

Synonyms

dNTP/dUTP Mixture | dNTP/dUTP Mix

Specifications & Purity

BioReagent, DNase, RNase free, PCR Reagent, Suitable for molecular biology, sterile, for DNA and RNA applications, ≥99%, dATP、dCTP、dGTP、dTTP: 2.5mM dUTP: 5mM

Stability And Storage

Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Storage

Store at -20°C, Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

This product requires cold chain shipping. Ground and other economy services are not available.

Grade

BioReagent, DNase, RNase free, for DNA and RNA applications, PCR Reagent, Sterile, Suitable for molecular biology

Purity

≥99%

Names and Identifiers

• PH: 7.0-7.5