Dissolve 52 grams per liter of distilled/deionized water, heating to boiling with stirring until completely solubilized.
Dispense into appropriate containers, loosen caps and autoclave for 15-30 minutes at 121ºC (15 psi).
Component
g/liter
Pancreatic Digest of Casein
17
Dextrose
2.5
Agar
12
Sodium Chloride
5
Papaic Digest of Soybean Meal
3
Potassium Phosphate, Dibasic
2.5
Tween 80
10
Total: 52g/Liter
Storage and Stability:
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
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