Simple identification experiments of plant peroxidases
Simple identification experiments of plant peroxidases
Peroxidases are metalloproteins containing iron in the porphyrin ring. Peroxidase forms a compound with hydrogen peroxide in which the hydrogen peroxide is activated so that it can oxidize phenolic compounds, acting as a receptor for hydrogen.
Operation method
Simple identification experiments of plant peroxidases
Principle
Peroxidases are metalloproteins containing iron in the porphyrin ring. Peroxidase forms a compound with hydrogen peroxide in which the hydrogen peroxide is activated so that it can oxidize phenolic compounds, acting as a receptor for hydrogen.
Materials and Instruments
Potato tubers Move Potato tubers, chopped, were added to two test tubes, one of which was boiled for a few minutes, and then 10 drops of freshly prepared 1.5% catechol alcoholic solution and 5 ml of 3% H2O2 solution were added to each, and if brown coloration occurred, the presence of peroxidase was indicated. For more product details, please visit Aladdin Scientific website.
Alcoholic solution of catechol H2O2
test tube
Take another test tube and add only H2O2 and catechol alcohol solution and observe the results.